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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
01/08/2013 |
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Data da última atualização: |
02/08/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SAWAZAKI, H. E.; SA, L. A. N. de; GONÇALVES, C. R. N. C. B.; VEIGA, R. F. A.; COLOMBO, C. A. |
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Afiliação: |
HAIKO ENOK SAWAZAKI, IAC; LUIZ ALEXANDRE NOGUEIRA DE SA, CNPMA; CASSIARA REGINA NOVENTA CORREA BARBOSA GONÇALVES, CTC; RENATO FERRAS DE ARRUDA VEIGA, IAC; CARLOS AUGUSTO COLOMBO, IAC. |
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Título: |
Molecular diagnosis optimization of virus, bacteria and fungi in sugarcane. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Internacional Research Journal of Plant Science, Sapele, v. 4, n. 3, p. 76-83, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Aiming at optimizing the diagnosis of major sugarcane diseases by PCR, primers were developed (scald, orange rust) and amplified fragments according to the literature were used as positive control, such as those caused by: 1 - Bacteria, (ratoon stunting and leaf scald) 2 - Viruses, (yellow leaf, mosaic, mosaic streak and fijivirus) 3-Fungus, (smut, orange rust and curvularia). For diseases with long latency period (leaf scald, ratoon stunting, yellow leaf, mosaic, fijivirus, smut and orange rust), primers were designed for real time PCR. For testing, infected leaves, fungal colonies, amplified DNA fragment or 40 seedlings were used. Real time PCR analyses have enabled the detection of sugarcane samples with highest dilution of DNA. The pair of primers designed for orange rust was more specific. The pair of primers designed for scald, apparently was not able to distinguish only bacteria of the genus Erwinia, whereas the primers described in the literature were observed amplify others genera, such as Xanthomonas, Pseudomonas, Erwinia, Stenotrophomona and Pantoea. The three strains of the fungus curvularia, found in sugarcane, resembled more to the species C.cymbopogonis, C.geniculata, C.sp. Of the 28 primer pairs tested, the best were shown for each of the disease-causing agent. |
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Palavras-Chave: |
Leaf scald; PCR. |
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Thesagro: |
Bactéria; Cana de açúcar; Diagnóstico; Doença de planta; Escaldadura; Ferrugem alaranjada; Fungo; Vírus. |
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Thesaurus Nal: |
Curvularia; Fijivirus; Plant diseases and disorders; Polymerase chain reaction; Ratoon stunting disease; Rust diseases; Scald diseases; Smut diseases; Sugarcane; Sugarcane streak virus; Sugarcane yellow leaf virus. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/86855/1/2013AP16.pdf
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Marc: |
LEADER 02511naa a2200421 a 4500
001 1963146
005 2013-08-02
008 2013 bl uuuu u00u1 u #d
100 1 $aSAWAZAKI, H. E.
245 $aMolecular diagnosis optimization of virus, bacteria and fungi in sugarcane.$h[electronic resource]
260 $c2013
520 $aAbstract: Aiming at optimizing the diagnosis of major sugarcane diseases by PCR, primers were developed (scald, orange rust) and amplified fragments according to the literature were used as positive control, such as those caused by: 1 - Bacteria, (ratoon stunting and leaf scald) 2 - Viruses, (yellow leaf, mosaic, mosaic streak and fijivirus) 3-Fungus, (smut, orange rust and curvularia). For diseases with long latency period (leaf scald, ratoon stunting, yellow leaf, mosaic, fijivirus, smut and orange rust), primers were designed for real time PCR. For testing, infected leaves, fungal colonies, amplified DNA fragment or 40 seedlings were used. Real time PCR analyses have enabled the detection of sugarcane samples with highest dilution of DNA. The pair of primers designed for orange rust was more specific. The pair of primers designed for scald, apparently was not able to distinguish only bacteria of the genus Erwinia, whereas the primers described in the literature were observed amplify others genera, such as Xanthomonas, Pseudomonas, Erwinia, Stenotrophomona and Pantoea. The three strains of the fungus curvularia, found in sugarcane, resembled more to the species C.cymbopogonis, C.geniculata, C.sp. Of the 28 primer pairs tested, the best were shown for each of the disease-causing agent.
650 $aCurvularia
650 $aFijivirus
650 $aPlant diseases and disorders
650 $aPolymerase chain reaction
650 $aRatoon stunting disease
650 $aRust diseases
650 $aScald diseases
650 $aSmut diseases
650 $aSugarcane
650 $aSugarcane streak virus
650 $aSugarcane yellow leaf virus
650 $aBactéria
650 $aCana de açúcar
650 $aDiagnóstico
650 $aDoença de planta
650 $aEscaldadura
650 $aFerrugem alaranjada
650 $aFungo
650 $aVírus
653 $aLeaf scald
653 $aPCR
700 1 $aSA, L. A. N. de
700 1 $aGONÇALVES, C. R. N. C. B.
700 1 $aVEIGA, R. F. A.
700 1 $aCOLOMBO, C. A.
773 $tInternacional Research Journal of Plant Science, Sapele$gv. 4, n. 3, p. 76-83, 2013.
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Embrapa Meio Ambiente (CNPMA) |
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