Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
07/07/2017 |
Data da última atualização: |
07/07/2017 |
Autoria: |
CHUNG, W. L.; PARISH, E. J.; BONE, L. W. |
Título: |
Sex steroid content and metabolism in Trichostrongylus colubriformis (Nematoda). |
Ano de publicação: |
1986 |
Fonte/Imprenta: |
The Journal of Parasitology, v. 72, n. 2, p. 326-329, Apr. 1986. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Testosterone, progesterone and cholesterol were found in mixed sexes of the nematode Trichostrongylus colubriformis from goats, according to thin-layer, gas-liquid and high-performance liquid chromatography. The structure of these steroids was confirmed by proton nuclear magnetic resonance and mass spectroscopy. Melting points of the worms' steroids were similar to authentic standards of the steroids. Estradiol was not detected in worms from either goat sex. Cholesterol was about 0.08% of the worms' dry weight in helminths from either sex of host. Testosterone was 0.02% of the dry weight when worms were taken from male goats, but only 0.005% from female goats. Progesterone was not detected in worms from male goats, but was 0.005% of the dry weight of helminths from female hosts. Incubation of a worm preparation with tritiated steroids showed that progesterone was converted to 17-alpha-hydroxyprogesterone, based on retention during radioactive thin-layer and gas-liquid chromatography, and co-crystallization. Testosterone, cholesterol and 17-beta-estradiol were not metabolized. |
Palavras-Chave: |
Host parasite relations. |
Thesaurus Nal: |
Cholesterol; Crystallization; Goats; Metabolism; Parasitism; Progesterone; Steroid hormones; Testosterone; Thin layer chromatography; Trichostrongylus colubriformis. |
Categoria do assunto: |
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Marc: |
LEADER 01883naa a2200277 a 4500 001 2072215 005 2017-07-07 008 1986 bl uuuu u00u1 u #d 100 1 $aCHUNG, W. L. 245 $aSex steroid content and metabolism in Trichostrongylus colubriformis (Nematoda). 260 $c1986 520 $aAbstract: Testosterone, progesterone and cholesterol were found in mixed sexes of the nematode Trichostrongylus colubriformis from goats, according to thin-layer, gas-liquid and high-performance liquid chromatography. The structure of these steroids was confirmed by proton nuclear magnetic resonance and mass spectroscopy. Melting points of the worms' steroids were similar to authentic standards of the steroids. Estradiol was not detected in worms from either goat sex. Cholesterol was about 0.08% of the worms' dry weight in helminths from either sex of host. Testosterone was 0.02% of the dry weight when worms were taken from male goats, but only 0.005% from female goats. Progesterone was not detected in worms from male goats, but was 0.005% of the dry weight of helminths from female hosts. Incubation of a worm preparation with tritiated steroids showed that progesterone was converted to 17-alpha-hydroxyprogesterone, based on retention during radioactive thin-layer and gas-liquid chromatography, and co-crystallization. Testosterone, cholesterol and 17-beta-estradiol were not metabolized. 650 $aCholesterol 650 $aCrystallization 650 $aGoats 650 $aMetabolism 650 $aParasitism 650 $aProgesterone 650 $aSteroid hormones 650 $aTestosterone 650 $aThin layer chromatography 650 $aTrichostrongylus colubriformis 653 $aHost parasite relations 700 1 $aPARISH, E. J. 700 1 $aBONE, L. W. 773 $tThe Journal of Parasitology$gv. 72, n. 2, p. 326-329, Apr. 1986.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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