01883naa a2200277 a 450000100080000000500110000800800410001910000170006024500850007726000090016252011070017165000160127865000200129465000100131465000150132465000150133965000170135465000210137165000170139265000300140965000350143965300280147470000180150270000160152077300690153620722152017-07-07       1986    bl uuuu     u00u1 u    #d1 aCHUNG, W. L.  aSex steroid content and metabolism in Trichostrongylus colubriformis (Nematoda).  c1986  aAbstract: Testosterone, progesterone and cholesterol were found in mixed sexes of the nematode Trichostrongylus colubriformis from goats, according to thin-layer, gas-liquid and high-performance liquid chromatography. The structure of these steroids was confirmed by proton nuclear magnetic resonance and mass spectroscopy. Melting points of the worms' steroids were similar to authentic standards of the steroids. Estradiol was not detected in worms from either goat sex. Cholesterol was about 0.08% of the worms' dry weight in helminths from either sex of host. Testosterone was 0.02% of the dry weight when worms were taken from male goats, but only 0.005% from female goats. Progesterone was not detected in worms from male goats, but was 0.005% of the dry weight of helminths from female hosts. Incubation of a worm preparation with tritiated steroids showed that progesterone was converted to 17-alpha-hydroxyprogesterone, based on retention during radioactive thin-layer and gas-liquid chromatography, and co-crystallization. Testosterone, cholesterol and 17-beta-estradiol were not metabolized.  aCholesterol  aCrystallization  aGoats  aMetabolism  aParasitism  aProgesterone  aSteroid hormones  aTestosterone  aThin layer chromatography  aTrichostrongylus colubriformis  aHost parasite relations1 aPARISH, E. J.1 aBONE, L. W.  tThe Journal of Parasitologygv. 72, n. 2, p. 326-329, Apr. 1986.