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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
21/02/2018 |
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Data da última atualização: |
21/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BIGI, M. M.; BLANCO, F. C.; ARAUJO, F. R.; THACKER, T. C.; ZUMÁRRAGA, M. J.; CATALDI, A. A.; SORIA, M. A.; BIGI, F. |
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Afiliação: |
María M. Bigi, School of Agronomy - UBA; Federico Carlos Blanco, Biotechnology Institute/National Institute of Agricultural Technology - INTA; FLABIO RIBEIRO ARAUJO, CNPGC; Tyler C. Thacker, United States Department of Agriculture/Agricultural Research Service/National Animal Disease Center; Martín J. Zumárraga, Biotechnology Institute/National Institute of Agricultural Technology - INTA; Angel A. Cataldi, Biotechnology Institute/National Institute of Agricultural Technology - INTA; Marcelo A. Soria, School of Agronomy - UBA; Fabiana Bigi, Biotechnology Institute/National Institute of Agricultural Technology - INTA. |
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Título: |
Polymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Microbiology and Immunology, v. 60, n. 8, p. 552-560, August 2016 |
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Idioma: |
Inglês |
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Conteúdo: |
Mycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans and animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and other members of the MTCevolved. The genome of M. bovis is over>99.95% identical to that of M. tuberculosis but with seven deletions ranging in size from 1 to 12.7 kb. In addition, 1200 single nucleotide mutations in coding regions distinguish M. bovis from M. tuberculosis. In the present study, we assessed 75 M. tuberculosis genomes and 23 M. bovis genomes to identify non-synonymous mutations in 202 coding sequences of regulatory genes between both species. We identified species-specific variants in 20 regulatory proteins and confirmed differential expression of hypoxia-related genes between M. bovis and M. tuberculosis. |
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Palavras-Chave: |
Regulator. |
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Thesagro: |
Anoxia; Mycobacterium Bovis; Polimorfismo. |
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Thesaurus Nal: |
Mycobacterium tuberculosis; Polymorphism. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/172939/1/27-Polymorphisms-of-20-regulatory-proteins-between-Mycobacterium-tuberculosis-and-Mycobacterium-bovis-2016.pdf
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Marc: |
LEADER 01718naa a2200277 a 4500
001 2087980
005 2018-02-21
008 2016 bl uuuu u00u1 u #d
100 1 $aBIGI, M. M.
245 $aPolymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis.$h[electronic resource]
260 $c2016
520 $aMycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans and animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and other members of the MTCevolved. The genome of M. bovis is over>99.95% identical to that of M. tuberculosis but with seven deletions ranging in size from 1 to 12.7 kb. In addition, 1200 single nucleotide mutations in coding regions distinguish M. bovis from M. tuberculosis. In the present study, we assessed 75 M. tuberculosis genomes and 23 M. bovis genomes to identify non-synonymous mutations in 202 coding sequences of regulatory genes between both species. We identified species-specific variants in 20 regulatory proteins and confirmed differential expression of hypoxia-related genes between M. bovis and M. tuberculosis.
650 $aMycobacterium tuberculosis
650 $aPolymorphism
650 $aAnoxia
650 $aMycobacterium Bovis
650 $aPolimorfismo
653 $aRegulator
700 1 $aBLANCO, F. C.
700 1 $aARAUJO, F. R.
700 1 $aTHACKER, T. C.
700 1 $aZUMÁRRAGA, M. J.
700 1 $aCATALDI, A. A.
700 1 $aSORIA, M. A.
700 1 $aBIGI, F.
773 $tMicrobiology and Immunology$gv. 60, n. 8, p. 552-560, August 2016
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
31/01/2019 |
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Data da última atualização: |
14/02/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
TORATI, L. S.; TAGGART, J. B.; VARELA, E. S.; ARARIPE, J.; WEHNER, S.; MIGAUD, H. |
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Afiliação: |
LUCAS SIMON TORATI, CNPASA; JOHN BERNARD TAGGART, UNIVERSITY OF STIRLING, Scotland-UK; EDUARDO SOUSA VARELA, CNPASA; JULIANA ARARIPE, UNIVERSIDADE FEDERAL DO PARÁ, Bragança-PA; STEFANIE WEHNER, MAX PLANCK INSTITUTE, Munich; HERVÉ MIGAUD, UNIVERSITY OF STIRLING, Scotland-UK. |
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Título: |
Genetic diversity and structure in Arapaima gigas populations from Amazon and Araguaia-Tocantins river basins. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
BMC Genetics, v. 20, n. 13, Jan. 2019. |
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ISSN: |
1471-2156 |
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DOI: |
10.1186/s12863-018-0711-y |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Arapaima gigas (Schinz, 1822) is the largest freshwater scaled fish in the world, and an emerging species for tropical aquaculture development. Conservation of the species, and the expansion of aquaculture requires the development of genetic tools to study polymorphism, differentiation, and stock structure. This study aimed to investigate genomic polymorphism through ddRAD sequencing, in order to identify a panel of single nucleotide polymorphisms (SNPs) and to simultaneously assess genetic diversity and structure in wild (from rivers Amazon, Solimões, Tocantins and Araguaia) and captive populations. Results: Compared to many other teleosts, the degree of polymorphism in A. gigas was low with only 2.3% of identified RAD-tags (135 bases long) containing SNPs. A panel of 393 informative SNPs was identified and screened across the five populations. Higher genetic diversity indices (number of polymorphic loci and private alleles, Shannon?s Index and HO) were found in populations from the Amazon and Solimões, intermediate levels in Tocantins and Captive, and very low levels in the Araguaia population. These results likely reflect larger population sizes from less urbanized environments in the Amazon basin compared to Araguaia. Populations were significantly differentiated with pairwise FST values ranging from 0.086 (Amazon × Solimões) to 0.556 (Amazon × Araguaia). Mean pairwise relatedness among individuals was significant in all populations (P < 0.01), reflecting a degree of inbreeding possibly due to severe depletion of natural stocks, the species sedentary behaviour and possible sampling biases. Although Mantel test was not significant (P = 0.104; R2 = 0.65), Bayesian analysis in STRUCTURE and discriminant analysis of principal components (DAPC) showed populations of Amazon and Solimões to be genetically differentiated from Araguaia, with Tocantins comprising individuals from both identified stocks. Conclusions: This relatively rapid genotyping by sequencing approach proved to be successful in delineating arapaima stocks. The approach and / or SNP panels identified should prove valuable for more detailed genetic studies of arapaima populations, including the elucidation of the genetic status of described discrete morphotypes and aid in delivery of conservation programs to maintain genetic diversity in reservoirs across the Amazon region. MenosBackground: Arapaima gigas (Schinz, 1822) is the largest freshwater scaled fish in the world, and an emerging species for tropical aquaculture development. Conservation of the species, and the expansion of aquaculture requires the development of genetic tools to study polymorphism, differentiation, and stock structure. This study aimed to investigate genomic polymorphism through ddRAD sequencing, in order to identify a panel of single nucleotide polymorphisms (SNPs) and to simultaneously assess genetic diversity and structure in wild (from rivers Amazon, Solimões, Tocantins and Araguaia) and captive populations. Results: Compared to many other teleosts, the degree of polymorphism in A. gigas was low with only 2.3% of identified RAD-tags (135 bases long) containing SNPs. A panel of 393 informative SNPs was identified and screened across the five populations. Higher genetic diversity indices (number of polymorphic loci and private alleles, Shannon?s Index and HO) were found in populations from the Amazon and Solimões, intermediate levels in Tocantins and Captive, and very low levels in the Araguaia population. These results likely reflect larger population sizes from less urbanized environments in the Amazon basin compared to Araguaia. Populations were significantly differentiated with pairwise FST values ranging from 0.086 (Amazon × Solimões) to 0.556 (Amazon × Araguaia). Mean pairwise relatedness among individuals was significant in all populations (P < 0.01), reflecting a d... Mostrar Tudo |
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Palavras-Chave: |
Araguaia; Tocantins. |
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Thesagro: |
Arapauma Gigas; Conservação; Genética Animal; Pirarucu. |
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Thesaurus NAL: |
Amazonia; Aquaculture; Arapaima gigas; Fisheries; Rivers; Species diversity. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/191822/1/CNPASA-2019-bmcgenetics.pdf
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Marc: |
LEADER 03345naa a2200349 a 4500
001 2105220
005 2020-02-14
008 2019 bl uuuu u00u1 u #d
022 $a1471-2156
024 7 $a10.1186/s12863-018-0711-y$2DOI
100 1 $aTORATI, L. S.
245 $aGenetic diversity and structure in Arapaima gigas populations from Amazon and Araguaia-Tocantins river basins.$h[electronic resource]
260 $c2019
520 $aBackground: Arapaima gigas (Schinz, 1822) is the largest freshwater scaled fish in the world, and an emerging species for tropical aquaculture development. Conservation of the species, and the expansion of aquaculture requires the development of genetic tools to study polymorphism, differentiation, and stock structure. This study aimed to investigate genomic polymorphism through ddRAD sequencing, in order to identify a panel of single nucleotide polymorphisms (SNPs) and to simultaneously assess genetic diversity and structure in wild (from rivers Amazon, Solimões, Tocantins and Araguaia) and captive populations. Results: Compared to many other teleosts, the degree of polymorphism in A. gigas was low with only 2.3% of identified RAD-tags (135 bases long) containing SNPs. A panel of 393 informative SNPs was identified and screened across the five populations. Higher genetic diversity indices (number of polymorphic loci and private alleles, Shannon?s Index and HO) were found in populations from the Amazon and Solimões, intermediate levels in Tocantins and Captive, and very low levels in the Araguaia population. These results likely reflect larger population sizes from less urbanized environments in the Amazon basin compared to Araguaia. Populations were significantly differentiated with pairwise FST values ranging from 0.086 (Amazon × Solimões) to 0.556 (Amazon × Araguaia). Mean pairwise relatedness among individuals was significant in all populations (P < 0.01), reflecting a degree of inbreeding possibly due to severe depletion of natural stocks, the species sedentary behaviour and possible sampling biases. Although Mantel test was not significant (P = 0.104; R2 = 0.65), Bayesian analysis in STRUCTURE and discriminant analysis of principal components (DAPC) showed populations of Amazon and Solimões to be genetically differentiated from Araguaia, with Tocantins comprising individuals from both identified stocks. Conclusions: This relatively rapid genotyping by sequencing approach proved to be successful in delineating arapaima stocks. The approach and / or SNP panels identified should prove valuable for more detailed genetic studies of arapaima populations, including the elucidation of the genetic status of described discrete morphotypes and aid in delivery of conservation programs to maintain genetic diversity in reservoirs across the Amazon region.
650 $aAmazonia
650 $aAquaculture
650 $aArapaima gigas
650 $aFisheries
650 $aRivers
650 $aSpecies diversity
650 $aArapauma Gigas
650 $aConservação
650 $aGenética Animal
650 $aPirarucu
653 $aAraguaia
653 $aTocantins
700 1 $aTAGGART, J. B.
700 1 $aVARELA, E. S.
700 1 $aARARIPE, J.
700 1 $aWEHNER, S.
700 1 $aMIGAUD, H.
773 $tBMC Genetics$gv. 20, n. 13, Jan. 2019.
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Embrapa Pesca e Aquicultura (CNPASA) |
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