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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
24/04/2021 |
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Data da última atualização: |
12/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TÁVORA, F. T. P. K.; BEVITORI, R.; MELLO, R. N. de; CINTRA, M. M. D. F.; OLIVEIRA NETO, O. B.; FONTES, W.; CASTRO, M. S.; SOUSA, M. V.; FRANCO, O. L.; REIS, A. M. dos. |
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Afiliação: |
FABIANO T. P. K. TÁVORA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA, MG; ROSANGELA BEVITORI, CNPAF; RAQUEL NEVES DE MELLO, CNPAF; MARIA MONICA DOMINGUES F CINTRA, CNPAF; OSMUNDO B. OLIVEIRA NETO, CENTRO UNIVERSITARIO UNIEURO, Brasília-DF; WAGNER FONTES, UNIVERSIDADE DE BRASÍLIA; MARIANA S. CASTRO, UNIVERSIDADE DE BRASÍLIA; MARCELO V. SOUSA, UNIVERSIDADE DE BRASÍLIA; OCTAVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANGELA MEHTA DOS REIS, Cenargen. |
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Título: |
Shotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Journal of Proteomics, v. 241, 104223, June 2021. |
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ISSN: |
1874-3919 |
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DOI: |
https://doi.org/10.1016/j.jprot.2021.104223 |
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Idioma: |
Inglês
Português |
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Notas: |
Na publicação: Angela Mehta. |
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Conteúdo: |
A comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance. MenosA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be furth... Mostrar Tudo |
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Palavras-Chave: |
Gene ontology; Plant immunity; PTO-based TIGS; RT-qPCR; S-gene; Transient-Induced gene silencing. |
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Thesagro: |
Arroz; Brusone; Doença de Planta; Oryza Sativa. |
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Thesaurus Nal: |
blast disease; disease resistance; foliar diseases; Magnaporthe oryzae; plant diseases and disorders; proteomics; rice. |
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Categoria do assunto: |
--
S Ciências Biológicas |
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Marc: |
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001 2131497
005 2021-07-12
008 2021 bl uuuu u00u1 u #d
022 $a1874-3919
024 7 $ahttps://doi.org/10.1016/j.jprot.2021.104223$2DOI
100 1 $aTÁVORA, F. T. P. K.
245 $aShotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance.$h[electronic resource]
260 $c2021
500 $aNa publicação: Angela Mehta.
520 $aA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance.
650 $ablast disease
650 $adisease resistance
650 $afoliar diseases
650 $aMagnaporthe oryzae
650 $aplant diseases and disorders
650 $aproteomics
650 $arice
650 $aArroz
650 $aBrusone
650 $aDoença de Planta
650 $aOryza Sativa
653 $aGene ontology
653 $aPlant immunity
653 $aPTO-based TIGS
653 $aRT-qPCR
653 $aS-gene
653 $aTransient-Induced gene silencing
700 1 $aBEVITORI, R.
700 1 $aMELLO, R. N. de
700 1 $aCINTRA, M. M. D. F.
700 1 $aOLIVEIRA NETO, O. B.
700 1 $aFONTES, W.
700 1 $aCASTRO, M. S.
700 1 $aSOUSA, M. V.
700 1 $aFRANCO, O. L.
700 1 $aREIS, A. M. dos
773 $tJournal of Proteomics$gv. 241, 104223, June 2021.
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Embrapa Arroz e Feijão (CNPAF) |
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| 9. |  | SIQUEIRA, K. B.; CARNEIRO, A. V.; ALMEIDA, M. F. de; HOTT, M. C.; GAMA, D. A. O mercado lácteo brasileiro no contexto mundial. In: STOCK, L. A.; ZOCCAL, R.; CARVALHO, G. R.; SIQUEIRA, K. B. (Ed.). Competitividade do agronegócio do leite brasileiro. Brasília, DF: Embrapa Informação Tecnológica; Juiz de Fora: Embrapa Gado de Leite, 2011. p. 13-33| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | SIQUEIRA, K. B.; ALMEIDA, M. F. de; STOCK, L. A.; DUARTE, M. G. P. Relações do preço do leite em pó integral no mercado internacional. In: CONGRESSO INTERNACIONAL DO LEITE, 8.; SIMPÓSIO SOBRE SUSTENTABILIDADE DA PECUÁRIA DE LEITE NO BRASIL, 10.; WORKSHOP SOBRE POLÍTICAS PÚBLICAS PARA O AGRONEGÓCIO DO LEITE, 8.; WORKSHOP DE QUALIDADE DO LEITE, 2., 2010, Juiz de Fora. Fórum das Américas: leite e derivados: anais. Juiz de Fora: Embrapa Gado de Leite, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | LINHARES, L. F.; SIQUEIRA, K. B.; ALMEIDA, M. F. de; HOTT, M. C.; FONSECA, L. d'A. M. Acordos de livre comércio: uma tendência mundial. Boletim CBLeite, Juiz de Fora, v. 5, n. 13, p. 73-78, 2011.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | PORTE, A.; TORQUILHO, H de S.; PENTEADO, A. L.; SOARES, N. B.; PORTE, L. H. M.; SOUZA, M. de; ALMEIDA, M. F. de. Composição química e atividade antimicrobiana de óleo essencial de Callistemon lanceolatus (Sm.) sweet. In: SIMPÓSIO DE ALIMENTOS E NUTRIÇÃO, 2., 2015, Rio de Janeiro. Caderno de Resumos... Rio de Janeiro: Universidade Federal do Estado do Rio de Janeiro, 2015. Ref. BT 009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 17 | |
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