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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/02/2013 |
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Data da última atualização: |
07/03/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CARVALHO, J. O.; MICHALCZECHEN-LACERDA, V. A.; SARTORI, R.; RODRIGUES, F. C.; BRAVIM, O.; FRANCO, M. M.; DODE, M. A. N. |
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Afiliação: |
JOSE O. CARVALHO, UNIVERSIDADE DE SÃO PAULO; VALQUIRIA A. MICHALCZECHEN-LACERDA, UnB; ROBERTO SARTORI, UNIVERSIDADE DE SÃO PAULO; FERNANDA C. RODRIGUES; OTAVIO BRAVIM, UnB; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
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Título: |
The methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Molecular Reproduction e Development, v. 79, p. 77-84, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted spermsamples fromfour Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed forX-sperm(SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70%Percoll gradient, and spermpellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisul?te sequencing.Methylation status of the IGF2 and IGF2Rgeneswere evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNAmethylation were found between NS, SX, and SY groups for the IGF2 (P¼0.09) or IGF2R genes (P¼0.38). Very speci?c methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methyla-tion patterns among bulls was observed. |
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Palavras-Chave: |
Gene IGF2; Gene IGF2R; Sexagem por citometria. |
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Thesagro: |
Bovino. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179337/1/Carvalho-et-al-2012-Molecular-Reproduction-and-Development.pdf
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Marc: |
LEADER 02081nam a2200229 a 4500
001 1950725
005 2023-03-07
008 2012 bl uuuu u00u1 u #d
100 1 $aCARVALHO, J. O.
245 $aThe methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting.$h[electronic resource]
260 $aMolecular Reproduction e Development, v. 79, p. 77-84, 2012.$c2012
520 $aThe objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted spermsamples fromfour Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed forX-sperm(SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70%Percoll gradient, and spermpellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisul?te sequencing.Methylation status of the IGF2 and IGF2Rgeneswere evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNAmethylation were found between NS, SX, and SY groups for the IGF2 (P¼0.09) or IGF2R genes (P¼0.38). Very speci?c methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methyla-tion patterns among bulls was observed.
650 $aBovino
653 $aGene IGF2
653 $aGene IGF2R
653 $aSexagem por citometria
700 1 $aMICHALCZECHEN-LACERDA, V. A.
700 1 $aSARTORI, R.
700 1 $aRODRIGUES, F. C.
700 1 $aBRAVIM, O.
700 1 $aFRANCO, M. M.
700 1 $aDODE, M. A. N.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
06/10/2003 |
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Data da última atualização: |
05/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PINEROS, M. A.; MAGALHAES, J. V.; ALVES, V. M. C.; KOCHIAN, L. V. |
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Afiliação: |
JURANDIR VIEIRA DE MAGALHAES, CNPMS; VERA MARIA CARVALHO ALVES, CNPMS. |
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Título: |
The physiology and biophysics of an aluminum tolerance mechanism based on root citrate exudation in maize. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Plant Physiology, Bethesda, v. 129, p. 1194-1206, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Al-induced release of Al-chelating ligands (primarily organic acids) into the rhizosphere from the root apex has been identified as a major Al tolerance mechanism in a number of plants species. In the present study, we conducted physiological investigations to study the spatial and temporal characteristcs of Al-activated root organic acid exudation, as well as changes in root organic acid content and Al accumulation, in an AL-tolerant maize (Zea mays) single cross (SLP 181/71 x Cateto Colombia 96/71). These investigations were integrated with biophysical studies using the patch-clamp technique to examine Al-activated anion channel activity in protoplasts isolated from different regions of the mazie root. Exposure to Al nearly instantaneously activated a concentration-dependent citrate release, which saturated at rates close to 0,5 nmol citrate h1 root -1. with the half-maximal rates of citrate release occurring at about 20 um Al3+ activity. Comparison of citrate exudation rates between decapped and capped roots indicated the root cap does not play a major role in perceiving the Al signal or in the exudation process. Spatial analysis indicated that the predominant citrate exudation is not confined to the root apex, but could be found as far as 5 cm beyond the root cap, involving cortex and stelar cells. Patch clamp recordings obtained in whole-cell and outside-out patches confirmed the presence of an Al-inducible plasma membrane anion channel in protoplasts isolated from stelar or cortical tissues. The unitary conductance of this channel was 23 to 55 pS. Our results suggest that this transporter mediates the Al-induced citrate release observed in the intact tissue. In addition to the rapid Al activation of citrate release , a slower, Al-inducible increase in root citrate content was also observed. These findings led us to speculate that in addition to the Al exclusion mechanism based on root citrate exudation, a second internal Al tolerance mechanism may be operating based on Al-inducible changes in organic acid synthesis and compartmentation. We discuss our findings in terms of recent genetic studies of Al tolerance in maize, which suggest that Al tolerance in maize is a complex trait. MenosAl-induced release of Al-chelating ligands (primarily organic acids) into the rhizosphere from the root apex has been identified as a major Al tolerance mechanism in a number of plants species. In the present study, we conducted physiological investigations to study the spatial and temporal characteristcs of Al-activated root organic acid exudation, as well as changes in root organic acid content and Al accumulation, in an AL-tolerant maize (Zea mays) single cross (SLP 181/71 x Cateto Colombia 96/71). These investigations were integrated with biophysical studies using the patch-clamp technique to examine Al-activated anion channel activity in protoplasts isolated from different regions of the mazie root. Exposure to Al nearly instantaneously activated a concentration-dependent citrate release, which saturated at rates close to 0,5 nmol citrate h1 root -1. with the half-maximal rates of citrate release occurring at about 20 um Al3+ activity. Comparison of citrate exudation rates between decapped and capped roots indicated the root cap does not play a major role in perceiving the Al signal or in the exudation process. Spatial analysis indicated that the predominant citrate exudation is not confined to the root apex, but could be found as far as 5 cm beyond the root cap, involving cortex and stelar cells. Patch clamp recordings obtained in whole-cell and outside-out patches confirmed the presence of an Al-inducible plasma membrane anion channel in protoplasts isolated from stel... Mostrar Tudo |
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Thesagro: |
Alumínio; Milho. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02777naa a2200181 a 4500
001 1487223
005 2018-06-05
008 2002 bl uuuu u00u1 u #d
100 1 $aPINEROS, M. A.
245 $aThe physiology and biophysics of an aluminum tolerance mechanism based on root citrate exudation in maize.$h[electronic resource]
260 $c2002
520 $aAl-induced release of Al-chelating ligands (primarily organic acids) into the rhizosphere from the root apex has been identified as a major Al tolerance mechanism in a number of plants species. In the present study, we conducted physiological investigations to study the spatial and temporal characteristcs of Al-activated root organic acid exudation, as well as changes in root organic acid content and Al accumulation, in an AL-tolerant maize (Zea mays) single cross (SLP 181/71 x Cateto Colombia 96/71). These investigations were integrated with biophysical studies using the patch-clamp technique to examine Al-activated anion channel activity in protoplasts isolated from different regions of the mazie root. Exposure to Al nearly instantaneously activated a concentration-dependent citrate release, which saturated at rates close to 0,5 nmol citrate h1 root -1. with the half-maximal rates of citrate release occurring at about 20 um Al3+ activity. Comparison of citrate exudation rates between decapped and capped roots indicated the root cap does not play a major role in perceiving the Al signal or in the exudation process. Spatial analysis indicated that the predominant citrate exudation is not confined to the root apex, but could be found as far as 5 cm beyond the root cap, involving cortex and stelar cells. Patch clamp recordings obtained in whole-cell and outside-out patches confirmed the presence of an Al-inducible plasma membrane anion channel in protoplasts isolated from stelar or cortical tissues. The unitary conductance of this channel was 23 to 55 pS. Our results suggest that this transporter mediates the Al-induced citrate release observed in the intact tissue. In addition to the rapid Al activation of citrate release , a slower, Al-inducible increase in root citrate content was also observed. These findings led us to speculate that in addition to the Al exclusion mechanism based on root citrate exudation, a second internal Al tolerance mechanism may be operating based on Al-inducible changes in organic acid synthesis and compartmentation. We discuss our findings in terms of recent genetic studies of Al tolerance in maize, which suggest that Al tolerance in maize is a complex trait.
650 $aAlumínio
650 $aMilho
700 1 $aMAGALHAES, J. V.
700 1 $aALVES, V. M. C.
700 1 $aKOCHIAN, L. V.
773 $tPlant Physiology, Bethesda$gv. 129, p. 1194-1206, 2002.
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