02777naa a2200181 a 450000100080000000500110000800800410001910000190006024501340007926000090021352022290022265000140245165000100246570000210247570000200249670000190251677300600253514872232018-06-05       2002    bl uuuu     u00u1 u    #d1 aPINEROS, M. A.  aThe physiology and biophysics of an aluminum tolerance mechanism based on root citrate exudation in maize.h[electronic resource]  c2002  aAl-induced release of Al-chelating ligands (primarily organic acids) into the rhizosphere from the root apex has been identified as a major Al tolerance mechanism in a number of plants species. In the present study, we conducted physiological investigations to study the spatial and temporal characteristcs of Al-activated root organic acid exudation, as well as changes in root organic acid content and Al accumulation, in an AL-tolerant maize (Zea mays) single cross (SLP 181/71 x Cateto Colombia 96/71). These investigations were integrated with biophysical studies using the patch-clamp technique to examine Al-activated anion channel activity in protoplasts isolated from different regions of the mazie root. Exposure to Al nearly instantaneously activated a concentration-dependent citrate release, which saturated at rates close to 0,5 nmol citrate h1 root -1. with the half-maximal rates of citrate release occurring at about 20 um Al3+ activity. Comparison of citrate exudation rates between decapped and capped roots indicated the root cap does not play a major role in perceiving the Al signal or in the exudation process. Spatial analysis indicated that the predominant citrate exudation is not confined to the root apex, but could be found as far as 5 cm beyond the root cap, involving cortex and stelar cells. Patch clamp recordings obtained in whole-cell and outside-out patches confirmed the presence of an Al-inducible plasma membrane anion channel in protoplasts isolated from stelar or cortical tissues. The unitary conductance of this channel was 23 to 55 pS. Our results suggest that this transporter mediates the Al-induced citrate release observed in the intact tissue. In addition to the rapid Al activation of citrate release , a slower, Al-inducible increase in root citrate content was also observed. These findings led us to speculate that in addition to the Al exclusion mechanism based on root citrate exudation, a second internal Al tolerance mechanism may be operating based on Al-inducible changes in organic acid synthesis and compartmentation. We discuss our findings in terms of recent genetic studies of Al tolerance in maize, which suggest that Al tolerance in maize is a complex trait.  aAlumínio  aMilho1 aMAGALHAES, J. V.1 aALVES, V. M. C.1 aKOCHIAN, L. V.  tPlant Physiology, Bethesdagv. 129, p. 1194-1206, 2002.