| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
13/04/2021 |
|
Data da última atualização: |
13/04/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SOUSA, T. P. de; CHAIBUB, A. A.; CORTES, M. V. de C. B.; BATISTA, T. F. C.; BEZERRA, G. de A.; SILVA, G. B. da; FILIPPI, M. C. C. de. |
|
Afiliação: |
THATYANE PEREIRA DE SOUSA, UFG; AMANDA ABDALLAH CHAIBUB, UFG; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; TELMA FATIMA COELHO BATISTA, UNIVERSIDADE FEDERAL RURAL DA AMAZONIA; GUSTAVO DE ANDRADE BEZERRA, UFG; GISELE BARATA DA SILVA, UNIVERSIDADE FEDERAL RURAL DA AMAZONIA; MARTA CRISTINA CORSI DE FILIPPI, CNPAF. |
|
Título: |
Molecular identification of Trichoderma sp. isolates and biochemical characterization of antagonistic interaction against rice blast. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Archives of Microbiology, 2021. |
|
ISSN: |
0302-8933 |
|
DOI: |
https://doi.org/10.1007/s00203-021-02307-5 |
|
Idioma: |
Inglês |
|
Conteúdo: |
This study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M. oryzae by mycoparasitism, and antibiosis mechanisms were interfered with by the M. oryzae infection process. MenosThis study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M... Mostrar Tudo |
|
Palavras-Chave: |
Antagonism; Bioagent; Mycoparasitism. |
|
Thesagro: |
Antagonismo; Arroz; Brusone; Controle Biológico; Marcador Molecular; Oryza Sativa; Trichoderma. |
|
Thesaurus Nal: |
Biological control; Mycoparasites; Rice. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
Marc: |
LEADER 02690naa a2200373 a 4500
001 2131247
005 2021-04-13
008 2021 bl uuuu u00u1 u #d
022 $a0302-8933
024 7 $ahttps://doi.org/10.1007/s00203-021-02307-5$2DOI
100 1 $aSOUSA, T. P. de
245 $aMolecular identification of Trichoderma sp. isolates and biochemical characterization of antagonistic interaction against rice blast.$h[electronic resource]
260 $c2021
520 $aThis study aimed to identify four isolates of Trichoderma sp. (Ufra.T06, Ufra.T09, Ufra.T12, and Ufra.T52) and characterize their interaction with Magnaporthe oryzae in vitro and in vivo conditions. The four isolates of Trichoderma sp. were sequenced, investigated as an antagonist against M. oryzae in five Petri plate assays, and as an inhibitor of conidial germination appressoria formation. Finally, were quantified the lytic activity of chitinase (CHI), glucanase (GLU), and protease (PRO) during co-cultivation of Trichoderma sp. and M. oryzae. In vivo, leaf blast suppression was evaluated intwo assays: simultaneous and curative application. Both in vitro and in vivo assays were scanned by electron microscopy (SEM). All isolates were identified as Trichoderma asperellum. All in vitro Petri plates assays reduced M. oryzae colony growth (paired?91.18% by Ufra.T09, volatile metabolites?all isolates equally reduced, non-volatile?68.33% by Ufra.T06,thermostability?99.77% by Ufra.T52 and co-cultivate?64.25% by Ufra.T52). The filtrates and conidia suspensions for T. asperellum isolates inhibited the conidia germination and appressoria formation significantly. In co-cultivate (mycelial or cell wall), all enzymes (GLU, CHI, and PRO) and times (24, 48, and 72 h) showed increased activity. In vivo, reduced leafblast severity until 94.64% (Ufra.T52cs) in a simultaneous and until 85% (Ufra.T09 24 and 48 hasi) in a curative application. T. asperellum isolates showed efficient control of M. oryzae by mycoparasitism, and antibiosis mechanisms were interfered with by the M. oryzae infection process.
650 $aBiological control
650 $aMycoparasites
650 $aRice
650 $aAntagonismo
650 $aArroz
650 $aBrusone
650 $aControle Biológico
650 $aMarcador Molecular
650 $aOryza Sativa
650 $aTrichoderma
653 $aAntagonism
653 $aBioagent
653 $aMycoparasitism
700 1 $aCHAIBUB, A. A.
700 1 $aCORTES, M. V. de C. B.
700 1 $aBATISTA, T. F. C.
700 1 $aBEZERRA, G. de A.
700 1 $aSILVA, G. B. da
700 1 $aFILIPPI, M. C. C. de
773 $tArchives of Microbiology, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
12/09/2008 |
|
Data da última atualização: |
16/06/2016 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
SILVA, E. M. S. da; FREITAS, B. M.; RIBEIRO, M. de F.; LOPES, T. M.; RIZZARDO, R. A. G. |
|
Afiliação: |
Eva Monica Sarmento da Silva, UNIVASF; Breno Magalhães Freitml, UFC; MARCIA DE FATIMA RIBEIRO, CPATSA; Thiago Mahlmann Lopes, UFC; Rômulo Augusto Guedes Rkzardo, UFC. |
|
Título: |
Visitantes florais do algodoeiro Gossypium hirsutum em Quixeramobim e Quixeré no Estado do Ceará. |
|
Ano de publicação: |
2008 |
|
Fonte/Imprenta: |
In: ENCONTRO SOBRE ABELHAS, 8., 2008, Ribeirão Preto. Biodiversidade e uso sustentado de abelhas: anais. Ribeirão Preto: FUNPEC, 2008. |
|
Descrição Física: |
1 CD-ROM. |
|
Idioma: |
Português |
|
Conteúdo: |
O objetivo deste estudo foi investigar os visitantes florais em áreas de algodoeiro comercial, cultivar 187 8R, nos municípios de Quixeramobim e Quixeré, Ceará. |
|
Palavras-Chave: |
Ceará; Diversidade de visitantes; Quixeramobim; Quixeré; Visitantes florais. |
|
Thesagro: |
Algodão; Polinização. |
|
Thesaurus NAL: |
Cotton; Pollination. |
|
Categoria do assunto: |
A Sistemas de Cultivo |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPATSA/38663/1/OPB1851.pdf
|
|
Marc: |
LEADER 01044nam a2200277 a 4500
001 1160188
005 2016-06-16
008 2008 bl uuuu u00u1 u #d
100 1 $aSILVA, E. M. S. da
245 $aVisitantes florais do algodoeiro Gossypium hirsutum em Quixeramobim e Quixeré no Estado do Ceará.$h[electronic resource]
260 $aIn: ENCONTRO SOBRE ABELHAS, 8., 2008, Ribeirão Preto. Biodiversidade e uso sustentado de abelhas: anais. Ribeirão Preto: FUNPEC$c2008
300 $c1 CD-ROM.
520 $aO objetivo deste estudo foi investigar os visitantes florais em áreas de algodoeiro comercial, cultivar 187 8R, nos municípios de Quixeramobim e Quixeré, Ceará.
650 $aCotton
650 $aPollination
650 $aAlgodão
650 $aPolinização
653 $aCeará
653 $aDiversidade de visitantes
653 $aQuixeramobim
653 $aQuixeré
653 $aVisitantes florais
700 1 $aFREITAS, B. M.
700 1 $aRIBEIRO, M. de F.
700 1 $aLOPES, T. M.
700 1 $aRIZZARDO, R. A. G.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
