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Registro Completo |
Biblioteca(s): |
Embrapa Meio-Norte. |
Data corrente: |
05/03/2020 |
Data da última atualização: |
12/12/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SANTOS, S. F. da C. B. dos; BRITO, L. de C. R. de; SILVA, G. S. F.; FERREIRA, A. C. M.; SILVA, A. D. de S.; SOUZA, H. A. de. |
Afiliação: |
SMAIELLO FLORES DA CONCEIÇÃO BORGES DOS SANTOS, UFPI; LUCÉLIA DE CÁSSIA RODRIGUES DE BRITO, UFPI; GABRIELA SABRINE FRANÇA SILVA, UFPI; ANE CAROLINE MELO FERREIRA, UFPI; ANTÔNIA DANIELA DE SOUSA SILVA, UFPI; HENRIQUE ANTUNES DE SOUZA, CPAMN. |
Título: |
Efeito do sombreamento de renques de eucalipto na produtividade da soja em sistema silviagrícola no Cerrado maranhense. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 4., 2018, Teresina, PI. Anais da IV Jornada Científica da Embrapa Meio-Norte. Brasília, DF: Embrapa, 2018. |
Páginas: |
p. 34. |
Idioma: |
Português |
Conteúdo: |
Objetivou-se avaliar os componentes de produção da soja, em função do sombreamento promovido pelos renques de eucalipto no Cerrado maranhense. |
Palavras-Chave: |
Consórcio de culturas; Sistemas integrados. |
Thesagro: |
Glycine Max. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/211552/1/Quarta-Jornada-Cientifica-2018-3-00032.pdf
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Marc: |
LEADER 00972nam a2200217 a 4500 001 2120972 005 2023-12-12 008 2018 bl uuuu u00u1 u #d 100 1 $aSANTOS, S. F. da C. B. dos 245 $aEfeito do sombreamento de renques de eucalipto na produtividade da soja em sistema silviagrícola no Cerrado maranhense.$h[electronic resource] 260 $aIn: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA MEIO-NORTE, 4., 2018, Teresina, PI. Anais da IV Jornada Científica da Embrapa Meio-Norte. Brasília, DF: Embrapa$c2018 300 $ap. 34. 520 $aObjetivou-se avaliar os componentes de produção da soja, em função do sombreamento promovido pelos renques de eucalipto no Cerrado maranhense. 650 $aGlycine Max 653 $aConsórcio de culturas 653 $aSistemas integrados 700 1 $aBRITO, L. de C. R. de 700 1 $aSILVA, G. S. F. 700 1 $aFERREIRA, A. C. M. 700 1 $aSILVA, A. D. de S. 700 1 $aSOUZA, H. A. de
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Embrapa Meio-Norte (CPAMN) |
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Registro Completo
Biblioteca(s): |
Embrapa Meio-Norte. |
Data corrente: |
19/01/2009 |
Data da última atualização: |
24/07/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
KOBAYASHI, A. K.; DIAZ TRUJILLO, C.; LEE, T. van der; ZWIERS, L.-H.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. |
Afiliação: |
ADILSON KENJI KOBAYASHI, CPAMN; CAUCASELLA DIAZ TRUJILLO, PLANT RESEARCH INTERNATIONAL; THEO VAN DER LEE; LUTE-HARM ZWIERS; LUCIANO V. PAIVA, FEDERAL UNIVERSITY OF LAVRAS; MANOEL TEIXEIRA SOUZA JUNIOR, EMBRAPA LABEX EUROPE; GERT H. J. KEMA, PLANT RESEARCH INTERNATIONAL. |
Título: |
Generation of transgenic Mycosphaerella fijiensis expressing reporter genes: tools for pathogenicity and mating studies. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland, 2008. |
Idioma: |
Inglês |
Conteúdo: |
Mycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducted in order to characterize the different stages in the pathogenesis on banana. MenosMycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducte... Mostrar Tudo |
Palavras-Chave: |
CIRAD139A; CIRAD86; DsRed; GFP. |
Thesagro: |
Doença; Fungo. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/69938/1/GenerationTransgenic-22676.pdf
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Marc: |
LEADER 02408nam a2200253 a 4500 001 1069938 005 2023-07-24 008 2008 bl uuuu u00u1 u #d 100 1 $aKOBAYASHI, A. K. 245 $aGeneration of transgenic Mycosphaerella fijiensis expressing reporter genes$btools for pathogenicity and mating studies.$h[electronic resource] 260 $aIn: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland$c2008 520 $aMycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducted in order to characterize the different stages in the pathogenesis on banana. 650 $aDoença 650 $aFungo 653 $aCIRAD139A 653 $aCIRAD86 653 $aDsRed 653 $aGFP 700 1 $aDIAZ TRUJILLO, C. 700 1 $aLEE, T. van der 700 1 $aZWIERS, L.-H. 700 1 $aPAIVA, L. V. 700 1 $aSOUZA JUNIOR, M. T. 700 1 $aKEMA, G. H. J.
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