02408nam a2200253 a 450000100080000000500110000800800410001910000210006024501480008126001570022952015820038665000120196865000100198065300140199065300120200465300100201665300080202670000220203470000200205670000180207670000170209470000240211170000190213510699382023-07-24       2008    bl uuuu     u00u1 u    #d1 aKOBAYASHI, A. K.  aGeneration of transgenic Mycosphaerella fijiensis expressing reporter genesbtools for pathogenicity and mating studies.h[electronic resource]  aIn: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerlandc2008  aMycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducted in order to characterize the different stages in the pathogenesis on banana.  aDoença  aFungo  aCIRAD139A  aCIRAD86  aDsRed  aGFP1 aDIAZ TRUJILLO, C.1 aLEE, T. van der1 aZWIERS, L.-H.1 aPAIVA, L. V.1 aSOUZA JUNIOR, M. T.1 aKEMA, G. H. J.