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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
02/07/1999 |
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Data da última atualização: |
20/05/2024 |
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Autoria: |
FINER, J. J.; KRIEBEL, H. B.; BECWAR, M. R. |
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Título: |
Initiation of embryogenic callus and suspension cultures of eastern white pine (Pinus strobus L.). |
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Ano de publicação: |
1989 |
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Fonte/Imprenta: |
Plant Cell Reports, Berlin, v. 8, n. 4, p. 203-206, 1989. |
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Idioma: |
Inglês |
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Conteúdo: |
Immature female gametophytes were placed on a medium containing 50 mg/litre glutamine, 500 mg/litre casein hydrolysate, 3% sucrose, 2 mg/litre 2,4-D, 1 mg/litre BA [benzyladenine] and 0.2% Gelrite as a solidifying agent. Cultures were initiated and maintained at 230C in the dark. Embryogenic calli could be seen as early as 5 days following culture. Histological studies indicated proliferation of pre-existing embryogenic tissue in the corrosion cavity followed by extrusion of embryogenic callus through the micropylar end of the gametophyte. Embryogenic suspension cultures were obtained by placing embryogenic callus into liquid medium. Suspension cultures were subcultured weekly and proliferated as early-stage embryos with attached suspensors. Embryo development was obtained following transfer of the embryogenic tissue to an auxin-free medium containing 50 mM glutamine, 38 5M ABA and 6% sucrose. Although embryo development could be consistently obtained, whole plants have not yet been recovered from these somatic embryos. |
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Palavras-Chave: |
2; 4 D; Cultura; Embriogenese somatica; Growth inhibitors; Pines; Plant growth regulators; Techniques. |
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Thesagro: |
Célula; Pinus Strobus; Tecido. |
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Thesaurus Nal: |
abscisic acid; benzyladenine; biotechnology; callus; conifers; cytokinins; growth regulators; tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02027naa a2200373 a 4500
001 1278588
005 2024-05-20
008 1989 bl uuuu u00u1 u #d
100 1 $aFINER, J. J.
245 $aInitiation of embryogenic callus and suspension cultures of eastern white pine (Pinus strobus L.).$h[electronic resource]
260 $c1989
520 $aImmature female gametophytes were placed on a medium containing 50 mg/litre glutamine, 500 mg/litre casein hydrolysate, 3% sucrose, 2 mg/litre 2,4-D, 1 mg/litre BA [benzyladenine] and 0.2% Gelrite as a solidifying agent. Cultures were initiated and maintained at 230C in the dark. Embryogenic calli could be seen as early as 5 days following culture. Histological studies indicated proliferation of pre-existing embryogenic tissue in the corrosion cavity followed by extrusion of embryogenic callus through the micropylar end of the gametophyte. Embryogenic suspension cultures were obtained by placing embryogenic callus into liquid medium. Suspension cultures were subcultured weekly and proliferated as early-stage embryos with attached suspensors. Embryo development was obtained following transfer of the embryogenic tissue to an auxin-free medium containing 50 mM glutamine, 38 5M ABA and 6% sucrose. Although embryo development could be consistently obtained, whole plants have not yet been recovered from these somatic embryos.
650 $aabscisic acid
650 $abenzyladenine
650 $abiotechnology
650 $acallus
650 $aconifers
650 $acytokinins
650 $agrowth regulators
650 $atissue culture
650 $aCélula
650 $aPinus Strobus
650 $aTecido
653 $a2
653 $a4 D
653 $aCultura
653 $aEmbriogenese somatica
653 $aGrowth inhibitors
653 $aPines
653 $aPlant growth regulators
653 $aTechniques
700 1 $aKRIEBEL, H. B.
700 1 $aBECWAR, M. R.
773 $tPlant Cell Reports, Berlin$gv. 8, n. 4, p. 203-206, 1989.
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Registro original: |
Embrapa Florestas (CNPF) |
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