02027naa a2200373 a 450000100080000000500110000800800410001910000170006024501260007726000090020352010400021265000180125265000180127065000180128865000110130665000130131765000150133065000220134565000190136765000120138665000180139865000110141665300060142765300080143365300120144165300260145365300220147965300100150165300280151165300150153970000190155470000180157377300620159112785882024-05-20       1989    bl uuuu     u00u1 u    #d1 aFINER, J. J.  aInitiation of embryogenic callus and suspension cultures of eastern white pine (Pinus strobus L.).h[electronic resource]  c1989  aImmature female gametophytes were placed on a medium containing 50 mg/litre glutamine, 500 mg/litre casein hydrolysate, 3% sucrose, 2 mg/litre 2,4-D, 1 mg/litre BA [benzyladenine] and 0.2% Gelrite as a solidifying agent. Cultures were initiated and maintained at 230C in the dark. Embryogenic calli could be seen as early as 5 days following culture. Histological studies indicated proliferation of pre-existing embryogenic tissue in the corrosion cavity followed by extrusion of embryogenic callus through the micropylar end of the gametophyte. Embryogenic suspension cultures were obtained by placing embryogenic callus into liquid medium. Suspension cultures were subcultured weekly and proliferated as early-stage embryos with attached suspensors. Embryo development was obtained following transfer of the embryogenic tissue to an auxin-free medium containing 50 mM glutamine, 38 5M ABA and 6% sucrose. Although embryo development could be consistently obtained, whole plants have not yet been recovered from these somatic embryos.  aabscisic acid  abenzyladenine  abiotechnology  acallus  aconifers  acytokinins  agrowth regulators  atissue culture  aCélula  aPinus Strobus  aTecido  a2  a4 D  aCultura  aEmbriogenese somatica  aGrowth inhibitors  aPines  aPlant growth regulators  aTechniques1 aKRIEBEL, H. B.1 aBECWAR, M. R.  tPlant Cell Reports, Berlingv. 8, n. 4, p. 203-206, 1989.