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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
17/09/2020 |
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Data da última atualização: |
16/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NERY, F. M. B.; MELO, F. L.; BOITEUX, L. S.; RIBEIRO, S. da G.; RESENDE, R. O.; ORÍLIO, A. F.; BATISTA, J. G.; LIMA, M. F.; PEREIRA-CARVALHO, R. C. |
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Afiliação: |
FLÁVIA M. B. NERY, UNIVERSIDADE DE BRASÍLIA; FERNANDO L. MELO, UNIVERSIDADE DE BRASÍLIA; LEONARDO SILVA BOITEUX, CNPH; SIMONE DA GRACA RIBEIRO, Cenargen; RENATO O. RESENDE, UNIVERSIDADE DE BRASÍLIA; ANELISE F. ORÍLIO, UNIVERSIDADE DE BRASÍLIA; JOSIANE G. BATISTA, UNIVERSIDADE DE BRASÍLIA; MIRTES FREITAS LIMA, CNPH; RITA C. PEREIRA-CARVALHO, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
Molecular characterization of Hovenia Dulcis-associated virus 1 (HDaV1) and 2 (HDaV2): new tentative species within the Order Picornavirales. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Viruses, v. 12, n. 9, 950, 2020 |
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DOI: |
https://doi.org/10.3390/v12090950 |
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Idioma: |
Inglês |
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Conteúdo: |
In a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered. MenosIn a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel viru... Mostrar Tudo |
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Palavras-Chave: |
HDaV1; HDaV2; Virome. |
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Thesagro: |
Hovenia Dulcis. |
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Thesaurus Nal: |
Metagenomics; Picornavirales; Viruses. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219297/1/ARTIGO-MolecularCharacterizationHoveniaDulcis.pdf
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Marc: |
LEADER 02445naa a2200313 a 4500
001 2128264
005 2020-12-16
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/v12090950$2DOI
100 1 $aNERY, F. M. B.
245 $aMolecular characterization of Hovenia Dulcis-associated virus 1 (HDaV1) and 2 (HDaV2)$bnew tentative species within the Order Picornavirales.$h[electronic resource]
260 $c2020
520 $aIn a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered.
650 $aMetagenomics
650 $aPicornavirales
650 $aViruses
650 $aHovenia Dulcis
653 $aHDaV1
653 $aHDaV2
653 $aVirome
700 1 $aMELO, F. L.
700 1 $aBOITEUX, L. S.
700 1 $aRIBEIRO, S. da G.
700 1 $aRESENDE, R. O.
700 1 $aORÍLIO, A. F.
700 1 $aBATISTA, J. G.
700 1 $aLIMA, M. F.
700 1 $aPEREIRA-CARVALHO, R. C.
773 $tViruses$gv. 12, n. 9, 950, 2020
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Registro original: |
Embrapa Hortaliças (CNPH) |
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Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
28/09/2012 |
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Data da última atualização: |
28/09/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
KROLOW, A. C. R.; PEREIRA, A. da S.; FERRI, N. M. L.; CORRÊA, A. P. A.; GONÇALVES, B. X.; ALVES, C. dos S. |
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Afiliação: |
ANA CRISTINA RICHTER KROLOW, CPACT; ARIONE DA SILVA PEREIRA, CPACT; NUBIA MARILIN LETTNIN FERRI, CPACT; ANA PAULA A. CORRÊA; BRENO X. GONÇALVES; CAMILA ALVES. |
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Título: |
Quantificação de açúcares redutores e matéria seca de batata cultivar asterix em diferentes temperaturas durante o armazenamento. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: CONGRESSO DE LA ASSOCIACIÓN LATINOAMERICANA DE LA PAPA, 25.; ENCONTTRO NACIONAL DE PRODUÇÃO E ABASTECIMENTO DE BATATA, 14., 2012, Uberlândia: Associación Latinoamericano de la Papa, 2012. |
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Idioma: |
Português |
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Thesagro: |
Batata. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/67121/1/Ana-Krolow-ALAP2012-0114publicado.pdf
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Marc: |
LEADER 00731nam a2200169 a 4500
001 1935036
005 2012-09-28
008 2012 bl uuuu u00u1 u #d
100 1 $aKROLOW, A. C. R.
245 $aQuantificação de açúcares redutores e matéria seca de batata cultivar asterix em diferentes temperaturas durante o armazenamento.$h[electronic resource]
260 $aIn: CONGRESSO DE LA ASSOCIACIÓN LATINOAMERICANA DE LA PAPA, 25.; ENCONTTRO NACIONAL DE PRODUÇÃO E ABASTECIMENTO DE BATATA, 14., 2012, Uberlândia: Associación Latinoamericano de la Papa$c2012
650 $aBatata
700 1 $aPEREIRA, A. da S.
700 1 $aFERRI, N. M. L.
700 1 $aCORRÊA, A. P. A.
700 1 $aGONÇALVES, B. X.
700 1 $aALVES, C. dos S.
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Embrapa Clima Temperado (CPACT) |
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