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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
19/09/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
MASCARENHAS, M. B.; PEIXOTO, P. V.; RAMADINHA, R. R.; ARMIEN, A. G.; COSTA, S. Z.; MIRANDA, I. C.; NOGUEIRA, V. A.; FRANÇA, T. N. |
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Afiliação: |
Mariana B. Mascarenhas, Programa de Pós-Graduação em Medicina Veterinária/Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro - UFRRJ; Paulo V. Peixoto, Departamento de Nutrição e Pastagem/Instituto de Zootecnia/Universidade Federal Rural do Rio de Janeiro - UFRRJ; Regina R. Ramadinha, Departamento de Medicina e Cirurgia Veterinária/Instituto de Veterinária/Universidade Federal Rural do Rio de Janeiro - UFRRJ; Anibal G. Armien, Veterinary Diagnostic Laboratory/Department of Veterinary Population Medicine/College of Veterinary Medicine/University of Minnesota; Samay Z. Costa, Programa de Pós-Graduação em Medicina Veterinária/Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro - UFRRJ; Ileana C. Miranda, Programa de Pós-Graduação em Medicina Veterinária/Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro - UFRRJ; Vivian A. Nogueira, Departamento de Epidemiologia e Saúde Pública/Instituto de Veterinária/Universidade Federal Rural do Rio de Janeiro - UFRRJ; Ticiana N. França, Departamento de Epidemiologia e Saúde Pública/Instituto de Veterinária/Universidade Federal Rural do Rio de Janeiro - UFRRJ. |
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Título: |
Immunohistochemical, lectin histochemical and ultrastructural studies of canine transmissible venereal tumor in Brazil |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 6, p. 613-620, jun. 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Estudos imuno-histoquímico, lectino-histoquímico e ultraestrutural do Tumor Venéreo Transmissível Canino no Brasil. |
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Conteúdo: |
Canine transmissible venereal tumor (CTVT) is a naturally occurring contagious roundcell neoplasia, with poorly understood origin and transmission. This study aims to further investigate the tumor nature through immunohistochemistry, lectin istochemistry and transmission electron microscopy (TEM) analysis, and to provide support for diagnostic and differential diagnoses of CTVT. Immunohistochemistry was performed in 10 genital and six exclusively extragenital tumors, which were previously diagnosed by citology and histopathology. CTVT samples were incubated with biotinylated antibodies to specific membrane and cytoplasmic antigens (anti-lysozyme, anti-macrophage, anti-vimentin, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3, polyclonal anti-CD117, polyclonal CD3 and anti-CD79a), followed by the avidin-biotin-peroxidase complex technique. The lectins Con A, DBA, SBA, PNA, UEA-1, WGA, sWGA, GSL, JSA, PSA, PHA-L, PHA-E and RCA were additionally tested in four genital CTVTs and TEM was performed in eight genital tumors. The anti-vimentin antibody revealed strong immunoreactivity to neoplastic cells in all the assessed samples (16/16). The polyclonal anti-CD3 antibodies showed moderate to strong immunoreactivity in fourteen (14/16) and the polyclonal anti-CD117 in fifteen cases (15/16). There was no immunoreactivity to anti-lysozyme, anti-macrophage, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3 and anti-CD79a antibodies. At lectin histochemistry, it was observed strong staining of tumor cells to Con-A, PHA-L and RCA. There was no histopathological and immunoreactivity differences between genital and extragenital CTVTs. These findings do not support the hypothesis of histiocytic origin of CTVT. In contrast, the lectin histochemical results were similar to cells from lymphoid/myeloid origin. MenosCanine transmissible venereal tumor (CTVT) is a naturally occurring contagious roundcell neoplasia, with poorly understood origin and transmission. This study aims to further investigate the tumor nature through immunohistochemistry, lectin istochemistry and transmission electron microscopy (TEM) analysis, and to provide support for diagnostic and differential diagnoses of CTVT. Immunohistochemistry was performed in 10 genital and six exclusively extragenital tumors, which were previously diagnosed by citology and histopathology. CTVT samples were incubated with biotinylated antibodies to specific membrane and cytoplasmic antigens (anti-lysozyme, anti-macrophage, anti-vimentin, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3, polyclonal anti-CD117, polyclonal CD3 and anti-CD79a), followed by the avidin-biotin-peroxidase complex technique. The lectins Con A, DBA, SBA, PNA, UEA-1, WGA, sWGA, GSL, JSA, PSA, PHA-L, PHA-E and RCA were additionally tested in four genital CTVTs and TEM was performed in eight genital tumors. The anti-vimentin antibody revealed strong immunoreactivity to neoplastic cells in all the assessed samples (16/16). The polyclonal anti-CD3 antibodies showed moderate to strong immunoreactivity in fourteen (14/16) and the polyclonal anti-CD117 in fifteen cases (15/16). There was no immunoreactivity to anti-lysozyme, anti-macrophage, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3 and anti-CD79a antibodies. At lectin histochemistry, it was observed s... Mostrar Tudo |
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Palavras-Chave: |
Canine transmissible venereal tumor; CTVT; Lectin histochemistry; Lectino-histoquímica; Tumor venéreo transmissível canino (TVTC); Ultrastructural. |
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Thesagro: |
Cão; Doença; Microscopia eletrônica. |
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Thesaurus Nal: |
Animal pathology; Dogs; Immunohistochemistry. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163939/1/Immunohistochemical-lectin-histochemical.pdf
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Marc: |
LEADER 03079naa a2200361 a 4500
001 2075805
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aMASCARENHAS, M. B.
245 $aImmunohistochemical, lectin histochemical and ultrastructural studies of canine transmissible venereal tumor in Brazil$h[electronic resource]
260 $c2017
500 $aTítulo em português: Estudos imuno-histoquímico, lectino-histoquímico e ultraestrutural do Tumor Venéreo Transmissível Canino no Brasil.
520 $aCanine transmissible venereal tumor (CTVT) is a naturally occurring contagious roundcell neoplasia, with poorly understood origin and transmission. This study aims to further investigate the tumor nature through immunohistochemistry, lectin istochemistry and transmission electron microscopy (TEM) analysis, and to provide support for diagnostic and differential diagnoses of CTVT. Immunohistochemistry was performed in 10 genital and six exclusively extragenital tumors, which were previously diagnosed by citology and histopathology. CTVT samples were incubated with biotinylated antibodies to specific membrane and cytoplasmic antigens (anti-lysozyme, anti-macrophage, anti-vimentin, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3, polyclonal anti-CD117, polyclonal CD3 and anti-CD79a), followed by the avidin-biotin-peroxidase complex technique. The lectins Con A, DBA, SBA, PNA, UEA-1, WGA, sWGA, GSL, JSA, PSA, PHA-L, PHA-E and RCA were additionally tested in four genital CTVTs and TEM was performed in eight genital tumors. The anti-vimentin antibody revealed strong immunoreactivity to neoplastic cells in all the assessed samples (16/16). The polyclonal anti-CD3 antibodies showed moderate to strong immunoreactivity in fourteen (14/16) and the polyclonal anti-CD117 in fifteen cases (15/16). There was no immunoreactivity to anti-lysozyme, anti-macrophage, anti-CD18, monoclonal anti-CD117, monoclonal anti-CD3 and anti-CD79a antibodies. At lectin histochemistry, it was observed strong staining of tumor cells to Con-A, PHA-L and RCA. There was no histopathological and immunoreactivity differences between genital and extragenital CTVTs. These findings do not support the hypothesis of histiocytic origin of CTVT. In contrast, the lectin histochemical results were similar to cells from lymphoid/myeloid origin.
650 $aAnimal pathology
650 $aDogs
650 $aImmunohistochemistry
650 $aCão
650 $aDoença
650 $aMicroscopia eletrônica
653 $aCanine transmissible venereal tumor
653 $aCTVT
653 $aLectin histochemistry
653 $aLectino-histoquímica
653 $aTumor venéreo transmissível canino (TVTC)
653 $aUltrastructural
700 1 $aPEIXOTO, P. V.
700 1 $aRAMADINHA, R. R.
700 1 $aARMIEN, A. G.
700 1 $aCOSTA, S. Z.
700 1 $aMIRANDA, I. C.
700 1 $aNOGUEIRA, V. A.
700 1 $aFRANÇA, T. N.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 6, p. 613-620, jun. 2017.
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Embrapa Unidades Centrais (AI-SEDE) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
23/03/2011 |
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Data da última atualização: |
03/05/2011 |
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Tipo da produção científica: |
Folder/Folheto/Cartilha |
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Autoria: |
AMARAL, T. B. |
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Afiliação: |
THAIS BASSO AMARAL, CNPGC. |
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Título: |
Manejo reprodutivo na pecuária de corte: fazendo certo! |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Campo Grande, MS: Embrapa Gado de Corte, 2010. |
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Descrição Física: |
1 Folder. |
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Idioma: |
Português |
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Notas: |
Foto da capa: Josimar Lima do Nascimento. Projeto gráfico: Luiz Leal, Paulo Paes e Darwin Augusto. |
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Conteúdo: |
Estação de monta. Época. Duração. Sistema de acasalamento. Relação touro/vacas. Diagnóstico de gestação e descartes. Touros. Doenças da esfera reprodutiva. Nutrição. Reposição de fêmeas. |
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Palavras-Chave: |
Monta; Pecuária de corte. |
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Thesagro: |
Reprodução Animal. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 00741nam a2200169 a 4500
001 1882278
005 2011-05-03
008 2010 bl uuuu u0uu1 u #d
100 1 $aAMARAL, T. B.
245 $aManejo reprodutivo na pecuária de corte$bfazendo certo!
260 $aCampo Grande, MS: Embrapa Gado de Corte$c2010
300 $c1 Folder.
500 $aFoto da capa: Josimar Lima do Nascimento. Projeto gráfico: Luiz Leal, Paulo Paes e Darwin Augusto.
520 $aEstação de monta. Época. Duração. Sistema de acasalamento. Relação touro/vacas. Diagnóstico de gestação e descartes. Touros. Doenças da esfera reprodutiva. Nutrição. Reposição de fêmeas.
650 $aReprodução Animal
653 $aMonta
653 $aPecuária de corte
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