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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
03/05/1999 |
Data da última atualização: |
03/05/1999 |
Autoria: |
ARAUJO, L. C. C. de. |
Título: |
Organizacao e metodos: integrando comportamento, estrutura, estrategia e tecnologia. |
Edição: |
2.ed. |
Ano de publicação: |
1989 |
Fonte/Imprenta: |
Sao Paulo: Atlas, 1989. |
Páginas: |
265p. |
Idioma: |
Português |
Palavras-Chave: |
Organizacao. |
Thesagro: |
Método. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00394nam a2200145 a 4500 001 1563698 005 1999-05-03 008 1989 bl uuuu 00u1 u #d 100 1 $aARAUJO, L. C. C. de 245 $aOrganizacao e metodos$bintegrando comportamento, estrutura, estrategia e tecnologia. 250 $a2.ed. 260 $aSao Paulo: Atlas$c1989 300 $a265p. 650 $aMétodo 653 $aOrganizacao
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Embrapa Cerrados (CPAC) |
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Registro Completo
Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Rondônia. |
Data corrente: |
08/02/2017 |
Data da última atualização: |
10/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
CAMPOS, F. T. de; RINCON, J. A. A.; ACOSTA, D. A. V.; SILVEIRA, P. A. S.; PRADIEÉ, J.; CORRÊA, M. N.; GASPERIN, B. G.; PFEIFER, L. F. M.; BARROS, C. C.; PEGORARO, L. M. C.; SCHNEIDER, A. |
Afiliação: |
Felipe Terres de Campos, UFPel; Joao Aveiro Alvarado Rincon, UFPel; Diego Andres Velasco Acosta, CORPOICA; Pedro Augusto Silva Silveira, UFPel; Jorgea Pradieé, Embrapa Clima Temperado; Marcio Nunes Corrêa, UFPel; Bernardo Garziera Gasperin, UFPel; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-Rondonia; Carlos Castilho Barros, UFPel; LIGIA MARGARETH CANTARELLI PEGORARO, CPACT; Augusto Schneider, UFPel. |
Título: |
The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Theriogenology, v. 89, p. 244 - 249, 2017. |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells. MenosThe aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic a... Mostrar Tudo |
Palavras-Chave: |
Esteroidogênese; PON1. |
Thesagro: |
Colesterol; Inflamação. |
Thesaurus NAL: |
cholesterol; inflammation; steroidogenesis. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227597/1/cpafro-17770.pdf
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Marc: |
LEADER 02572naa a2200325 a 4500 001 2063230 005 2021-11-10 008 2017 bl uuuu u00u1 u #d 100 1 $aCAMPOS, F. T. de 245 $aThe acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.$h[electronic resource] 260 $c2017 520 $aThe aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells. 650 $acholesterol 650 $ainflammation 650 $asteroidogenesis 650 $aColesterol 650 $aInflamação 653 $aEsteroidogênese 653 $aPON1 700 1 $aRINCON, J. A. A. 700 1 $aACOSTA, D. A. V. 700 1 $aSILVEIRA, P. A. S. 700 1 $aPRADIEÉ, J. 700 1 $aCORRÊA, M. N. 700 1 $aGASPERIN, B. G. 700 1 $aPFEIFER, L. F. M. 700 1 $aBARROS, C. C. 700 1 $aPEGORARO, L. M. C. 700 1 $aSCHNEIDER, A. 773 $tTheriogenology$gv. 89, p. 244 - 249, 2017.
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