02572naa a2200325 a 450000100080000000500110000800800410001910000210006024502020008126000090028352015900029265000160188265000170189865000200191565000150193565000170195065300210196765300090198870000210199770000210201870000230203970000170206270000190207970000200209870000220211870000180214070000230215870000180218177300470219920632302021-11-10       2017    bl uuuu     u00u1 u    #d1 aCAMPOS, F. T. de  aThe acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.h[electronic resource]  c2017  aThe aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P &lt 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P &lt 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P &lt 0.05). The FF E2 and P4 concentrations were not different between groups (P &gt 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P &lt 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells.  acholesterol  ainflammation  asteroidogenesis  aColesterol  aInflamação  aEsteroidogênese  aPON11 aRINCON, J. A. A.1 aACOSTA, D. A. V.1 aSILVEIRA, P. A. S.1 aPRADIEÉ, J.1 aCORRÊA, M. N.1 aGASPERIN, B. G.1 aPFEIFER, L. F. M.1 aBARROS, C. C.1 aPEGORARO, L. M. C.1 aSCHNEIDER, A.  tTheriogenologygv. 89, p. 244 - 249, 2017.