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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
26/12/2001 |
Data da última atualização: |
14/01/2020 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
FARIA, J. C.; ALBINO, M. M. C.; DIAS, B. B. A.; VIANNA, M. G. R.; ARAGÃO, F. J. L. |
Afiliação: |
JOSIAS CORREA DE FARIA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen. |
Título: |
Feijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado. |
Ano de publicação: |
2001 |
Fonte/Imprenta: |
In: CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOTECNOLOGIA, 1., 2001, São Paulo. [Resumos expandidos]... Brasilia: SBBIOTEC, 2001. |
Idioma: |
Português |
Conteúdo: |
Tbe rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication, and rep gene rnutations with amino acid codons cbanges in the putative NTP-binding rnotif D262R (MI) was created. Phaseolus vulgaris rransforrnation was achieved with a helium driven particle acceleration device. Tbe vector contained the bar gene [resistance to arnmonium glyphosinate (AG)] and besides the rep gene mutant. A total of J 7 initial - R(O) - transformants were analyzed. From the R(O) plant M1/4, which had mutation MI, six progeny plants with resistance to AG were obtained, and four remained BGMV symptomless after inoculation. From tbese four plants, a total progeny of 127 R(2) plants were inoculated. Of the 35 progenies of plant M 1/4/l, only three plants showed syrnptoms. From the other 92 plants, 72 had symptoms. Two plants out of 20 in the nontransgenic control plants remained symptornless. |
Palavras-Chave: |
BGMV; Biobalística; Tolerância; Transformação. |
Thesagro: |
Feijão; Mosaico Dourado; Phaseolus Vulgaris; Vírus. |
Thesaurus Nal: |
Bean golden mosaic virus; mutation. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208710/1/feijoeiro-transgenico0001.pdf
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Marc: |
LEADER 01804nam a2200277 a 4500 001 1209188 005 2020-01-14 008 2001 bl uuuu u00u1 u #d 100 1 $aFARIA, J. C. 245 $aFeijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado.$h[electronic resource] 260 $aIn: CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOTECNOLOGIA, 1., 2001, São Paulo. [Resumos expandidos]... Brasilia: SBBIOTEC$c2001 520 $aTbe rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication, and rep gene rnutations with amino acid codons cbanges in the putative NTP-binding rnotif D262R (MI) was created. Phaseolus vulgaris rransforrnation was achieved with a helium driven particle acceleration device. Tbe vector contained the bar gene [resistance to arnmonium glyphosinate (AG)] and besides the rep gene mutant. A total of J 7 initial - R(O) - transformants were analyzed. From the R(O) plant M1/4, which had mutation MI, six progeny plants with resistance to AG were obtained, and four remained BGMV symptomless after inoculation. From tbese four plants, a total progeny of 127 R(2) plants were inoculated. Of the 35 progenies of plant M 1/4/l, only three plants showed syrnptoms. From the other 92 plants, 72 had symptoms. Two plants out of 20 in the nontransgenic control plants remained symptornless. 650 $aBean golden mosaic virus 650 $amutation 650 $aFeijão 650 $aMosaico Dourado 650 $aPhaseolus Vulgaris 650 $aVírus 653 $aBGMV 653 $aBiobalística 653 $aTolerância 653 $aTransformação 700 1 $aALBINO, M. M. C. 700 1 $aDIAS, B. B. A. 700 1 $aVIANNA, M. G. R. 700 1 $aARAGÃO, F. J. L.
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Embrapa Arroz e Feijão (CNPAF) |
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