01804nam a2200277 a 450000100080000000500110000800800410001910000170006024501590007726001310023652009050036765000290127265000130130165000120131465000200132665000230134665000110136965300090138065300180138965300160140765300200142370000210144370000190146470000210148370000220150412091882020-01-14       2001    bl uuuu     u00u1 u    #d1 aFARIA, J. C.  aFeijoeiro (Phaseolus vulgaris L.) transgênico contendo um mutante do gene REP apresenta resistência ao vírus do mosaico dourado.h[electronic resource]  aIn: CONGRESSO DA SOCIEDADE BRASILEIRA DE BIOTECNOLOGIA, 1., 2001, São Paulo. [Resumos expandidos]... Brasilia: SBBIOTECc2001  aTbe rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication, and rep gene rnutations with amino acid codons cbanges in the putative NTP-binding rnotif D262R (MI) was created. Phaseolus vulgaris rransforrnation was achieved with a helium driven particle acceleration device. Tbe vector contained the bar gene [resistance to arnmonium glyphosinate (AG)] and besides the rep gene mutant. A total of J 7 initial - R(O) - transformants were analyzed. From the R(O) plant M1/4, which had mutation MI, six progeny plants with resistance to AG were obtained, and four remained BGMV symptomless after inoculation. From tbese four plants, a total progeny of 127 R(2) plants were inoculated. Of the 35 progenies of plant M 1/4/l, only three plants showed syrnptoms. From the other 92 plants, 72 had symptoms. Two plants out of 20 in the nontransgenic control plants remained symptornless.  aBean golden mosaic virus  amutation  aFeijão  aMosaico Dourado  aPhaseolus Vulgaris  aVírus  aBGMV  aBiobalística  aTolerância  aTransformação1 aALBINO, M. M. C.1 aDIAS, B. B. A.1 aVIANNA, M. G. R.1 aARAGÃO, F. J. L.