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Registro Completo |
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Rondônia. |
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Data corrente: |
08/02/2017 |
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Data da última atualização: |
10/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMPOS, F. T. de; RINCON, J. A. A.; ACOSTA, D. A. V.; SILVEIRA, P. A. S.; PRADIEÉ, J.; CORRÊA, M. N.; GASPERIN, B. G.; PFEIFER, L. F. M.; BARROS, C. C.; PEGORARO, L. M. C.; SCHNEIDER, A. |
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Afiliação: |
Felipe Terres de Campos, UFPel; Joao Aveiro Alvarado Rincon, UFPel; Diego Andres Velasco Acosta, CORPOICA; Pedro Augusto Silva Silveira, UFPel; Jorgea Pradieé, Embrapa Clima Temperado; Marcio Nunes Corrêa, UFPel; Bernardo Garziera Gasperin, UFPel; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-Rondonia; Carlos Castilho Barros, UFPel; LIGIA MARGARETH CANTARELLI PEGORARO, CPACT; Augusto Schneider, UFPel. |
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Título: |
The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Theriogenology, v. 89, p. 244 - 249, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells. MenosThe aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic a... Mostrar Tudo |
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Palavras-Chave: |
Esteroidogênese; PON1. |
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Thesagro: |
Colesterol; Inflamação. |
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Thesaurus Nal: |
cholesterol; inflammation; steroidogenesis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227597/1/cpafro-17770.pdf
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Marc: |
LEADER 02572naa a2200325 a 4500
001 2063230
005 2021-11-10
008 2017 bl uuuu u00u1 u #d
100 1 $aCAMPOS, F. T. de
245 $aThe acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.$h[electronic resource]
260 $c2017
520 $aThe aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells.
650 $acholesterol
650 $ainflammation
650 $asteroidogenesis
650 $aColesterol
650 $aInflamação
653 $aEsteroidogênese
653 $aPON1
700 1 $aRINCON, J. A. A.
700 1 $aACOSTA, D. A. V.
700 1 $aSILVEIRA, P. A. S.
700 1 $aPRADIEÉ, J.
700 1 $aCORRÊA, M. N.
700 1 $aGASPERIN, B. G.
700 1 $aPFEIFER, L. F. M.
700 1 $aBARROS, C. C.
700 1 $aPEGORARO, L. M. C.
700 1 $aSCHNEIDER, A.
773 $tTheriogenology$gv. 89, p. 244 - 249, 2017.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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| Registros recuperados : 9 | |
| 1. |  | ACOSTA, D. A. V.; JACOMETO, C. B.; SCHMITT, E.; PEREIRA, R. A.; DEL PINO, F. A. B.; CORREA, M. N. Efeito de dietas contendo diferentes proporções de AGPIS no ganho de peso de ratos em sucessivas gerações. In: ENCONTRO DE PÓS GRADUAÇÃO UFPEL, 15., 2012, Pelotas - RS. Anais... Pelotas: UFPel, 2012.| Biblioteca(s): Embrapa Rondônia. |
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| 2. | | SILVA, J. F. da; RINCÓN, J. A. A; CAMPOS, F. T. de; ACOSTA, D. A. V.; PEGORARO, L. M. C.; SCHNEIDER, A. Atividade da paraoxonase-1 na fração hdl no soro e no fluido folicular de vacas jersey desafiadas com lipopolisacarideos. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 25.; SEMANA INTEGRADA DE ENSINO, PESQUISA E EXTENSÃO, 2., 2016, Pelotas. Anais... Pelotas: UFPel, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 3. | | RINCÓN, J. A. A.; CAMPOS, F. T. DE; ACOSTA, D. A.; SILVEIRA, P. A. S.; GASPERIN, B. G.; CORREA, M. N.; PEGORARO, L. M. C.; SCHNEIDER, A. The effect of lipopolysaccharides on the expression of genes involved in steroidogenesis and inflammatory response in bovine granulosa cells. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Publicado na revista Animal Reproduction, v. 13, n. 3, p. 529, Jul./Sept. 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 4. | | RINCÓN, J. A. A.; MION, B.; ACOSTA, D. A. V.; GASPERIN, B. G.; ROVANI, M. T.; PEGORARO, L. M. C.; CORRÊA, M. N.; SCHNEIDER, A. Effect of recombinant bovine somatotropin (rbST) treatment on follicular population and development in non-lactating dairy cows. Animal Reproduction, v. 16, n. 4. p. 1-9, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 5. | | RINCÓN, J. A. A.; CAMPOS, F. T. de; ACOSTA, D. A. V.; SILVEIRA, P. A.; PEGORARO, L. M. C.; SCHNEIDER, A. Efeito do LPS sobre a expressão gênica em células da granulosa do folículo dominante de bovinos. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 24.; ENCONTRO DE PÓS-GRADUAÇÃO, 17., 2015, Pelotas. [Anais.]. Pelotas: UFPel, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 6. | | ACOSTA, D. A.; RINCÓN, J. A. A.; SILVEIRA, P. A. S.; PRADIEÉ, J.; CORREA, M. N.; PEGORARO, L. M. C.; SCHNEIDER, A. Effects of somatotropin injection on gene expression of follicular cells from the dominant follicle in cows. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Publicado na revista Animal Reproduction, v. 13, n. 3, p. 531, Jul./Sept. 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 7. | | CAMPOS, F. T. de; RINCON, J. A. A.; ACOSTA, D. A. V.; SILVEIRA, P. A. S.; PRADIEÉ, J.; CORRÊA, M. N.; GASPERIN, B. G.; PFEIFER, L. F. M.; BARROS, C. C.; PEGORARO, L. M. C.; SCHNEIDER, A. The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle. Theriogenology, v. 89, p. 244 - 249, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Clima Temperado; Embrapa Rondônia. |
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| 8. | | ACOSTA, D. A. V.; PFEIFER, L. F. M.; SCHMITT, E.; SCHNEIDER, A.; SILVEIRA, P. A. S.; JACOMETO, C. B.; BRAUNER, C. C.; RABASSA, R. V.; CORRÊA, M. N.; PINO, F. A. B. D. Effect of prepartum somatotropin injection in late pregnant Holstein heifers with high body condition score on metabolic parameters, resumption of ovulation and milk production. Canadian Journal of Animal Science, Ottawa, v. 93, p. 287-292, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Rondônia. |
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| 9. | | SCHWEGLER, E.; SCHNEIDER, A.; MONTAGNER, P.; ACOSTA, D. A. V.; PFEIFER, L. F. M.; SCHMITT, E.; RABASSA, V. R.; DEL PINO, F. A. B.; GONZALEZ, H. de L.; TIMM, C. D.; CORRÊA, M. N. Predictive value of prepartum serum metabolites for incidence of clinical and subclinical mastitis in grazing primiparous Holstein cows. Tropical Animal Health and Production, Edinburgh, v. 45, n. 3, mar. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Rondônia. |
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| Registros recuperados : 9 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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