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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
24/07/2012 |
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Data da última atualização: |
11/09/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FOLLONI, S.; KAGKLI, D.-M.; RAJCEVIC, B.; GUIMARAES, N. C. C.; VAN DROOGENBROECK, B.; VALICENTE, F. H.; EEDE, G. V. D.; BULCKE, M. V. D. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
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Título: |
Detection of airborne genetically modified maize pollen by real-time PCR. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Molecular Ecology Resources, Oxford, v. 12, n. 5, p. 810-821, 2012. |
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DOI: |
10.1111/j.1755-0998.2012.03168.x |
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Idioma: |
Inglês |
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Conteúdo: |
The cultivation of genetically modified (GM) crops has raised numerous concerns in the European Union and other parts of the world about their environmental and economic impact. Especially outcrossing of genetically modified organisms (GMO) was from the beginning a critical issue as airborne pollen has been considered an important way of GMO dis- persal. Here, we investigate the use of airborne pollen sampling combined with microscopic analysis and molecular PCR analysis as an approach to monitor GMmaize cultivations in a specific area. Field trial experiments in the European Union and South America demonstrated the applicability of the approach under different climate conditions, in rural and semi-urban environment, even at very low levels of airborne pollen. The study documents in detail the sampling of GMpollen, sample DNA extraction and real-time PCR analysis. Our results suggest that this ?GM pollen monitoring by bioaerosol sampling and PCR screening? approachmight represent an useful aid in the surveillance of GM-free areas, centres of origin and natural reserves. |
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Palavras-Chave: |
Bioaerosol; Genetically modified organism. |
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Thesagro: |
Milho; Organismo transgenico. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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001 1929002
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008 2012 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1755-0998.2012.03168.x$2DOI
100 1 $aFOLLONI, S.
245 $aDetection of airborne genetically modified maize pollen by real-time PCR.$h[electronic resource]
260 $c2012
520 $aThe cultivation of genetically modified (GM) crops has raised numerous concerns in the European Union and other parts of the world about their environmental and economic impact. Especially outcrossing of genetically modified organisms (GMO) was from the beginning a critical issue as airborne pollen has been considered an important way of GMO dis- persal. Here, we investigate the use of airborne pollen sampling combined with microscopic analysis and molecular PCR analysis as an approach to monitor GMmaize cultivations in a specific area. Field trial experiments in the European Union and South America demonstrated the applicability of the approach under different climate conditions, in rural and semi-urban environment, even at very low levels of airborne pollen. The study documents in detail the sampling of GMpollen, sample DNA extraction and real-time PCR analysis. Our results suggest that this ?GM pollen monitoring by bioaerosol sampling and PCR screening? approachmight represent an useful aid in the surveillance of GM-free areas, centres of origin and natural reserves.
650 $aMilho
650 $aOrganismo transgenico
653 $aBioaerosol
653 $aGenetically modified organism
700 1 $aKAGKLI, D.-M.
700 1 $aRAJCEVIC, B.
700 1 $aGUIMARAES, N. C. C.
700 1 $aVAN DROOGENBROECK, B.
700 1 $aVALICENTE, F. H.
700 1 $aEEDE, G. V. D.
700 1 $aBULCKE, M. V. D.
773 $tMolecular Ecology Resources, Oxford$gv. 12, n. 5, p. 810-821, 2012.
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Embrapa Milho e Sorgo (CNPMS) |
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| 1. |  | GALÃO, O. F.; BORSATO, D.; PINTO, J. P.; PINA, MARCOS V. R.; VISENTAINER, J. V.; PANIZZI, M. C.; LEITE, R. S. Aplicação de redes neurais artificiais na classificação e identificação de cultivares de soja por região de plantio. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 33., 2010, Águas de Lindóia. A química construindo um futuro melhor. [São Paulo]: SBQ, 2010. Ref. BEA-014. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
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