01888naa a2200265 a 450000100080000000500110000800800410001902400420006010000160010224501010011826000090021952010910022865000100131965000260132965300150135565300340137070000180140470000170142270000240143970000260146370000210148970000190151070000210152977300720155019290022012-09-11       2012    bl uuuu     u00u1 u    #d7 a10.1111/j.1755-0998.2012.03168.x2DOI1 aFOLLONI, S.  aDetection of airborne genetically modified maize pollen by real-time PCR.h[electronic resource]  c2012  aThe cultivation of genetically modified (GM) crops has raised numerous concerns in the European Union and other parts of the world about their environmental and economic impact. Especially outcrossing of genetically modified organisms (GMO) was from the beginning a critical issue as airborne pollen has been considered an important way of GMO dis- persal. Here, we investigate the use of airborne pollen sampling combined with microscopic analysis and molecular PCR analysis as an approach to monitor GMmaize cultivations in a specific area. Field trial experiments in the European Union and South America demonstrated the applicability of the approach under different climate conditions, in rural and semi-urban environment, even at very low levels of airborne pollen. The study documents in detail the sampling of GMpollen, sample DNA extraction and real-time PCR analysis. Our results suggest that this ?GM pollen monitoring by bioaerosol sampling and PCR screening? approachmight represent an useful aid in the surveillance of GM-free areas, centres of origin and natural reserves.  aMilho  aOrganismo transgenico  aBioaerosol  aGenetically modified organism1 aKAGKLI, D.-M.1 aRAJCEVIC, B.1 aGUIMARAES, N. C. C.1 aVAN DROOGENBROECK, B.1 aVALICENTE, F. H.1 aEEDE, G. V. D.1 aBULCKE, M. V. D.  tMolecular Ecology Resources, Oxfordgv. 12, n. 5, p. 810-821, 2012.