Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
11/12/2000 |
Data da última atualização: |
04/08/2023 |
Autoria: |
HO, G.; CAMPBELL, W. H.; BERGDOLL, M. S.; CARLSON, E. |
Título: |
Production of a toxic shock syndrome toxin variant by Staphylococcus aureus strains associated with sheep, goats, and cows. |
Ano de publicação: |
1989 |
Fonte/Imprenta: |
Journal of Clinical Microbiology, v. 27, n. 9, p. 1946-1948, Sep. 1989. |
DOI: |
https://doi.org/10.1128/jcm.27.9.1946-1948.1989 |
Idioma: |
Inglês |
Conteúdo: |
A toxic shock syndrome toxin (TSST) variant with an isoelectric point (pI) of 8.6 produced by an ovine-associated Staphylococcus aureus strain was described previously. Analysis of additional strains associated with sheep, goats, cows, and humans by isoelectric focusing with immunoblotting using monoclonal antibodies revealed that all 18 strains associated with sheep and all 12 strains associated with goats produced the TSST variant. Only 1 of 10 bovine-associated strains and no human-associated strains produced the variant, whereas the others produced TSST-1 (pI between 7.0 and 7.2). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis with immunoblotting indicated that both TSST-1 and the TSST variant had a molecular size of 24 kilodaltons. |
Palavras-Chave: |
Mastite. |
Thesagro: |
Staphylococcus Aureus. |
Thesaurus Nal: |
Bacterial toxins; Bovine mastitis; Cattle diseases; Enterotoxins; Goat diseases; Goats; Microbiology; Molecular weight; Polyacrylamide gel electrophoresis; Sheep diseases; Western blotting. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 01769naa a2200325 a 4500 001 1524184 005 2023-08-04 008 1989 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1128/jcm.27.9.1946-1948.1989$2DOI 100 1 $aHO, G. 245 $aProduction of a toxic shock syndrome toxin variant by Staphylococcus aureus strains associated with sheep, goats, and cows.$h[electronic resource] 260 $c1989 520 $aA toxic shock syndrome toxin (TSST) variant with an isoelectric point (pI) of 8.6 produced by an ovine-associated Staphylococcus aureus strain was described previously. Analysis of additional strains associated with sheep, goats, cows, and humans by isoelectric focusing with immunoblotting using monoclonal antibodies revealed that all 18 strains associated with sheep and all 12 strains associated with goats produced the TSST variant. Only 1 of 10 bovine-associated strains and no human-associated strains produced the variant, whereas the others produced TSST-1 (pI between 7.0 and 7.2). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis with immunoblotting indicated that both TSST-1 and the TSST variant had a molecular size of 24 kilodaltons. 650 $aBacterial toxins 650 $aBovine mastitis 650 $aCattle diseases 650 $aEnterotoxins 650 $aGoat diseases 650 $aGoats 650 $aMicrobiology 650 $aMolecular weight 650 $aPolyacrylamide gel electrophoresis 650 $aSheep diseases 650 $aWestern blotting 650 $aStaphylococcus Aureus 653 $aMastite 700 1 $aCAMPBELL, W. H. 700 1 $aBERGDOLL, M. S. 700 1 $aCARLSON, E. 773 $tJournal of Clinical Microbiology$gv. 27, n. 9, p. 1946-1948, Sep. 1989.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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