01769naa a2200325 a 450000100080000000500110000800800410001902400570006010000110011724501510012826000090027952007620028865000210105065000200107165000200109165000170111165000180112865000100114665000170115665000210117365000390119465000190123365000210125265000260127365300120129970000200131170000200133170000160135177300760136715241842023-08-04       1989    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1128/jcm.27.9.1946-1948.19892DOI1 aHO, G.  aProduction of a toxic shock syndrome toxin variant by Staphylococcus aureus strains associated with sheep, goats, and cows.h[electronic resource]  c1989  aA toxic shock syndrome toxin (TSST) variant with an isoelectric point (pI) of 8.6 produced by an ovine-associated Staphylococcus aureus strain was described previously. Analysis of additional strains associated with sheep, goats, cows, and humans by isoelectric focusing with immunoblotting using monoclonal antibodies revealed that all 18 strains associated with sheep and all 12 strains associated with goats produced the TSST variant. Only 1 of 10 bovine-associated strains and no human-associated strains produced the variant, whereas the others produced TSST-1 (pI between 7.0 and 7.2). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis with immunoblotting indicated that both TSST-1 and the TSST variant had a molecular size of 24 kilodaltons.  aBacterial toxins  aBovine mastitis  aCattle diseases  aEnterotoxins  aGoat diseases  aGoats  aMicrobiology  aMolecular weight  aPolyacrylamide gel electrophoresis  aSheep diseases  aWestern blotting  aStaphylococcus Aureus  aMastite1 aCAMPBELL, W. H.1 aBERGDOLL, M. S.1 aCARLSON, E.  tJournal of Clinical Microbiologygv. 27, n. 9, p. 1946-1948, Sep. 1989.