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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/07/2001 |
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Data da última atualização: |
10/05/2023 |
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Autoria: |
ANTHONY, P.; DAVEVY, M. R.; POWER, J. B.; LOWE, K. C. |
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Afiliação: |
Department of Life Science, University of Nottingham, University Park, Nottingham NG7 2RD, UK. |
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Título: |
An improved protocol for the culture of cassava leaf protoplasts. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Plant Cell., v.42, p.299-302, 1995. |
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Idioma: |
Inglês |
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Conteúdo: |
Viable protoplasts (yield > 1.9 x 10 g-1 fresh weight; mean viability 85= 2%, n=5) were isolated from leaves of axenix shoot cultures of Manihot esculents Crantz. cv. M. Thai 8. Protoplasts were cultured for up to 50 days in liquid, ammonium-free MS medium, overlaying agarose-solidified B5 medium with short glass rods embedded perpendicularly within, and prodruting from, the agarose layer. Control protoplasts were cultured identically, but without glass rods. Sustained protoplasts division was observed only in the presence of glass rods, where the initial planting efficiency was almost 6-fold greater than control (p < 0.05). The mean final plating efficiency of treated culture was 1.0 = 0.2% while, in contrast, significant colony formation was not observed in controls. |
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Thesagro: |
Mandioca. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01240naa a2200169 a 4500
001 1650182
005 2023-05-10
008 1995 bl uuuu u00u1 u #d
100 1 $aANTHONY, P.
245 $aAn improved protocol for the culture of cassava leaf protoplasts.$h[electronic resource]
260 $c1995
520 $aViable protoplasts (yield > 1.9 x 10 g-1 fresh weight; mean viability 85= 2%, n=5) were isolated from leaves of axenix shoot cultures of Manihot esculents Crantz. cv. M. Thai 8. Protoplasts were cultured for up to 50 days in liquid, ammonium-free MS medium, overlaying agarose-solidified B5 medium with short glass rods embedded perpendicularly within, and prodruting from, the agarose layer. Control protoplasts were cultured identically, but without glass rods. Sustained protoplasts division was observed only in the presence of glass rods, where the initial planting efficiency was almost 6-fold greater than control (p < 0.05). The mean final plating efficiency of treated culture was 1.0 = 0.2% while, in contrast, significant colony formation was not observed in controls.
650 $aMandioca
700 1 $aDAVEVY, M. R.
700 1 $aPOWER, J. B.
700 1 $aLOWE, K. C.
773 $tPlant Cell.$gv.42, p.299-302, 1995.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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