01240naa a2200169 a 450000100080000000500110000800800410001910000160006024500930007626000090016952007880017865000130096670000180097970000170099770000160101477300400103016501822023-05-10       1995    bl uuuu     u00u1 u    #d1 aANTHONY, P.  aAn improved protocol for the culture of cassava leaf protoplasts.h[electronic resource]  c1995  aViable protoplasts (yield &gt 1.9 x 10 g-1 fresh weight; mean viability 85= 2%, n=5) were isolated from leaves of axenix shoot cultures of Manihot esculents Crantz. cv. M. Thai 8. Protoplasts were cultured for up to 50 days in liquid, ammonium-free MS medium, overlaying agarose-solidified B5 medium with short glass rods embedded perpendicularly within, and prodruting from, the agarose layer. Control protoplasts were cultured identically, but without glass rods. Sustained protoplasts division was observed only in the presence of glass rods, where the initial planting efficiency was almost 6-fold greater than control (p &lt 0.05). The mean final plating efficiency of treated culture was 1.0 = 0.2% while, in contrast, significant colony formation was not observed in controls.  aMandioca1 aDAVEVY, M. R.1 aPOWER, J. B.1 aLOWE, K. C.  tPlant Cell.gv.42, p.299-302, 1995.