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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/04/1995 |
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Data da última atualização: |
17/05/2023 |
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Autoria: |
KOBAYASHI, S.; OHGAWARA, T.; OHGAWARA, E.; OIYAMA, I.; ISHII, S. |
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Afiliação: |
Akitsu Branch, Fruit Tree Rsearch Station, Akitsu, Hiroshima, Japao; Research & Development Division, Kikkoman Corporation, Noda-shi, China. |
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Título: |
A somatic hybrid plant obtained by protoplast fusion between navel orange (Citrus sinensis) and satsuma mandarin (C. unshiu). |
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Ano de publicação: |
1988 |
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Fonte/Imprenta: |
Plant Cell, tissue and Organ Culture, v. 14, p.63-69 1988. |
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Idioma: |
Inglês |
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Conteúdo: |
A somatic hybrid plant was obtained by protoplast fusion between navelorange and satsuma mandarin. Protoplasts isolated from nucellar calli of navel orange and from leaves of satsuma mandari were fused by the PEG method. The fusion products were cultured in a Murashige & Tucker medium containing 0.6M sucrose. In this medium, some colonies developedinto whole plants thorough embryogenesis. One of the regenerated plants was shown to be a hybrid, which was proven by restriction endonuclease analysis of nuclear ribosomal DNA. The chromosome number of the hybrid was 36. Both parents have a chromosome number 2n = 18. |
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Palavras-Chave: |
rDNA analysis; Somatic hybrid plant. |
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Thesaurus Nal: |
Citrus; protoplast fusion. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01283naa a2200217 a 4500
001 1646453
005 2023-05-17
008 1988 bl uuuu u00u1 u #d
100 1 $aKOBAYASHI, S.
245 $aA somatic hybrid plant obtained by protoplast fusion between navel orange (Citrus sinensis) and satsuma mandarin (C. unshiu).$h[electronic resource]
260 $c1988
520 $aA somatic hybrid plant was obtained by protoplast fusion between navelorange and satsuma mandarin. Protoplasts isolated from nucellar calli of navel orange and from leaves of satsuma mandari were fused by the PEG method. The fusion products were cultured in a Murashige & Tucker medium containing 0.6M sucrose. In this medium, some colonies developedinto whole plants thorough embryogenesis. One of the regenerated plants was shown to be a hybrid, which was proven by restriction endonuclease analysis of nuclear ribosomal DNA. The chromosome number of the hybrid was 36. Both parents have a chromosome number 2n = 18.
650 $aCitrus
650 $aprotoplast fusion
653 $arDNA analysis
653 $aSomatic hybrid plant
700 1 $aOHGAWARA, T.
700 1 $aOHGAWARA, E.
700 1 $aOIYAMA, I.
700 1 $aISHII, S.
773 $tPlant Cell, tissue and Organ Culture$gv. 14, p.63-69 1988.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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