01283naa a2200217 a 450000100080000000500110000800800410001910000180006024501530007826000090023152006230024065000110086365000220087465300180089665300250091470000170093970000170095670000150097370000140098877300630100216464532023-05-17       1988    bl uuuu     u00u1 u    #d1 aKOBAYASHI, S.  aA somatic hybrid plant obtained by protoplast fusion between navel orange (Citrus sinensis) and satsuma mandarin (C. unshiu).h[electronic resource]  c1988  aA somatic hybrid plant was obtained by protoplast fusion between navelorange and satsuma mandarin. Protoplasts isolated from nucellar calli of navel orange and from leaves of satsuma mandari were fused by the PEG method. The fusion products were cultured in a Murashige & Tucker medium containing 0.6M sucrose. In this medium, some colonies developedinto whole plants thorough embryogenesis. One of the regenerated plants was shown to be a hybrid, which was proven by restriction endonuclease analysis of nuclear ribosomal DNA. The chromosome number of the hybrid was 36. Both parents have a chromosome number 2n = 18.  aCitrus  aprotoplast fusion  arDNA analysis  aSomatic hybrid plant1 aOHGAWARA, T.1 aOHGAWARA, E.1 aOIYAMA, I.1 aISHII, S.  tPlant Cell, tissue and Organ Culturegv. 14, p.63-69 1988.