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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
13/03/2014 |
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Data da última atualização: |
19/10/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FARIA, M. T. de; CURY-BOAVENTURA, M. F.; LOPES, L. R.; SILVA, J. R. M. C. da. |
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Afiliação: |
MARCOS TUCUNDUVA DE FARIA, CPATU; Maria Fernanda Cury-Boaventura, USP; Lucia Rossetti Lopes, USP; José Roberto Machado Cunha da Silva, USP. |
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Título: |
Generation of reactive oxygen species by leukocytes of Prochilodus lineatus. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Fish Physiology and Biochemistry, v. 40, n. 2, p. 445-455, Apr. 2014. |
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DOI: |
10.1007/s10695-013-9856-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Prochilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 −, using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47phox and p67phox). Thus, catalase enhanced the presence of p47phox. Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the respiratory burst pathways in this species and suggests mitochondria as the organelle responsible for generation of reactive oxygen species. MenosProchilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 −, using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47phox and p67phox). Thus, catalase enhanced the presence of p47phox. Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the r... Mostrar Tudo |
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Palavras-Chave: |
Citometria de fluxo; Espécies reativas de oxigênio; Explosão respiratória; Mitocôndrias. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02340naa a2200217 a 4500
001 1982284
005 2022-10-19
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s10695-013-9856-9$2DOI
100 1 $aFARIA, M. T. de
245 $aGeneration of reactive oxygen species by leukocytes of Prochilodus lineatus.$h[electronic resource]
260 $c2014
520 $aProchilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 −, using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47phox and p67phox). Thus, catalase enhanced the presence of p47phox. Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the respiratory burst pathways in this species and suggests mitochondria as the organelle responsible for generation of reactive oxygen species.
653 $aCitometria de fluxo
653 $aEspécies reativas de oxigênio
653 $aExplosão respiratória
653 $aMitocôndrias
700 1 $aCURY-BOAVENTURA, M. F.
700 1 $aLOPES, L. R.
700 1 $aSILVA, J. R. M. C. da
773 $tFish Physiology and Biochemistry$gv. 40, n. 2, p. 445-455, Apr. 2014.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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| Registros recuperados : 11 | |
| 5. |  | RAMOS, D. M.; VALLS, J. F. M.; OLIVEIRA, R. C. de; GRACIANO RIBEIRO, D. A New Awned Species of Paspalum (Poaceae, Panicoideae, Paniceae) from Brazil. Novon, v. 21, n. 3, p. 368-372, Sept. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | COSTA, L. C.; WONDRACEK, D. C.; ROCHA, R. A.; RAMOS, D. M.; VALLS, J. F. M. Stigma tip morphology of Arachis (Fabaceae) species from seven taxonomic sections, with emphasis on sections Extranervosae and Heteranthae. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 59| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | RAMOS, D. M.; FERREIRA, D. N. M.; VALLS, J. F. M. Teratologia causada por ácaros do gênero Aceria keifer (Acari: Prostigmata: Eriophyidae) Emespiguetas de Panicum Olyroides kunth (Poaceae). In: CONGRESSO NACIONAL DE BOTÂNICA, 60.; REUNIÃO NORDESTINA DE BOTÂNICA, 32.; ENCONTRO REGIONAL DE BOTÂNICOS - MG, BA , ES, 29., 2009, Feira de Santana. Botânica brasileira: futuro e compromissos. Feira de Santana: SBB: UEFS, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | RAMOS, D. M.; SILVA, F. de L. e; FUJIYAMA, V. B.; BORGHETTI, F.; VALLS, J. F. M. Temporal changes in seed dormancy, germination and viability of grasses from neotropical savannas. In: CONGRESSO NACIONAL DE BOTÂNICA, 67., 2016, Vitória, ES. Conectando diversidades, revelando o desconhecido: resumos. Brasília, DF: Sociedade Botânica do Brasil, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | ROCHA, R. A.; COSTA, L. C.; WONDRACEK, D. C.; CUSTODIO, A. R.; RAMOS, D. M.; VALLS, J. F. M. Variation in seedling morphology of wild Arachis species. In: INTERNATIONAL CONFERENCE OF THE PEANUT RESEARCH COMMUNITY ON ADVANCES IN ARACHIS THROUGH GENOMICS AND BIOTECNOLOGY, 5., 2011, Brasília, DF. Book of abstracts... Brasília, DF: Embrapa Genetic Researces and Biotecnology, 2011. p. 58| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | RAMOS, D. M.; ANDRADE, J. M.; ALBERTON, B. C.; MOURA, M. S. B. de; DOMINGUES, T. F.; NEVES, N.; LIMA, J. R. S.; SOUZA, R.; SOUZA, E.; SILVA, J. R.; SANTO, M. M. E.; MORELLATO, L. P. C.; CUNHA, J. Multiscale phenology of seasonally dry tropical forests in an aridity gradient. Frontiers in Environmental Sciience, v. 11, 1275844, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Semiárido. |
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| 11. | | MOONLIGHT, P. W.; BANDA-R, K.; PHILLIPS, O. L.; DEXTER, K. G.; PENNINGTON, R. T.; BAKER, T. R.; LIMA, H. C. de; FAJARDO, L.; GONZALEZ-M., R.; PALOMINO, R. L.; LLOYD, L.; NASCIMENTO, M.; PRADO, D.; QUINTANA, C.; RIINA, R.; RODRIGUEZ M. G. M.; VILLELA, D. M.; AQUINO, A. C. M. M.; ARROYO, L.; BEZERRA, C.; BRUNELLO, A. T.; BRIENEN, R. J. W.; CARDOSO, D.; CHAO, K.-J.; COUTINHO, I. A. C.; CUNHA, J.; DOMINGUES, T.; SANTO, M. M. do E.; FELDPAUSCH, T. R.; FERNANDES, M. F.; GOODWIN, Z. A.; JIMENEZ, E. M.; LEVESLEY, A.; TOLEDO, L. L.; MARIMON, B.; MIATTO, R. C.; MIZUSHIMA, M.; MONTEAGUDO, A.; MOURA, M. S. B. de; MURAKAMI, A.; NEVES, D.; CHEQUIN, R. N.; OLIVEIRA, T. C. de S.; OLIVEIRA, E. A. de; QUEIROZ, L. P. de; PILON, A.; RAMOS, D. M.; REYNEL, C.; RODRIGUES, P. M. S.; SANTOS, R.; SARKINEN, T.; SILVA, V. F. da; SOUZA, R. M. S.; VASQUEZ, R.; VEENENDAAL, E. Expanding tropical forest monitoring into dry forests: the DRYFLOR protocol for permanent plots. Plants People Planet, v. 3, n. 3, p. 295-300, 2021.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 11 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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