|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
26/03/1996 |
|
Data da última atualização: |
05/12/2023 |
|
Autoria: |
McNAMARA, P. J.; BRADLY, G. A.; SONGER, J. G. |
|
Título: |
Targeted mutagenesis of the phospholipase d gene results in decreased virulence of Corynebacterium pseudotuberculosis. |
|
Ano de publicação: |
1994 |
|
Fonte/Imprenta: |
Molecular Microbiologia, v. 12, n. 6, p. 921-930, Jun. 1994. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: The chromosomal gene encoding the phospholipase D from Corynebacterium pseudotuberculosis (biovar ovis) isolate Whetten 1 was replaced with an allele containing a nonsense mutation. The virulence of the mutant strain (W1.31r1) and the isogenic parental strain were then compared by inoculation of goats. The wild-type strain caused abscessation at the site of infection, which then spread to the regional lymph node, while W1.31r1 had a reduced ability to establish a primary infection and was incapable of dissemination. Our results confirm that phospholipase D is a virulence determinant of C. pseudotuberculosis that increases the persistence and spread of the bacteria within the host. |
|
Palavras-Chave: |
Corybacterium pseudotuberculosis; Microbiologia molecular. |
|
Thesagro: |
Corynebacterium Pseudotuberculosis; Linfadenite Caseosa. |
|
Thesaurus Nal: |
Amino acid sequences; Enzymology; Gene targeting; Goat diseases; Goats; Microbiology; Mutagenesis; Mutation; Phospholipase D; Physiology; Virulence. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01676naa a2200325 a 4500
001 1522010
005 2023-12-05
008 1994 bl uuuu u00u1 u #d
100 1 $aMcNAMARA, P. J.
245 $aTargeted mutagenesis of the phospholipase d gene results in decreased virulence of Corynebacterium pseudotuberculosis.$h[electronic resource]
260 $c1994
520 $aAbstract: The chromosomal gene encoding the phospholipase D from Corynebacterium pseudotuberculosis (biovar ovis) isolate Whetten 1 was replaced with an allele containing a nonsense mutation. The virulence of the mutant strain (W1.31r1) and the isogenic parental strain were then compared by inoculation of goats. The wild-type strain caused abscessation at the site of infection, which then spread to the regional lymph node, while W1.31r1 had a reduced ability to establish a primary infection and was incapable of dissemination. Our results confirm that phospholipase D is a virulence determinant of C. pseudotuberculosis that increases the persistence and spread of the bacteria within the host.
650 $aAmino acid sequences
650 $aEnzymology
650 $aGene targeting
650 $aGoat diseases
650 $aGoats
650 $aMicrobiology
650 $aMutagenesis
650 $aMutation
650 $aPhospholipase D
650 $aPhysiology
650 $aVirulence
650 $aCorynebacterium Pseudotuberculosis
650 $aLinfadenite Caseosa
653 $aCorybacterium pseudotuberculosis
653 $aMicrobiologia molecular
700 1 $aBRADLY, G. A.
700 1 $aSONGER, J. G.
773 $tMolecular Microbiologia$gv. 12, n. 6, p. 921-930, Jun. 1994.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
