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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
02/05/2014 |
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Data da última atualização: |
10/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOURA, R. R.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; MELO, C. H. S.; SANCHEZ, D. J. D.; VIEIRA, M. P.; ALMEIDA, T. M.; SEROVA, I. A.; SEROV, O. L.; PEREIRA, A. F.; TEIXEIRA, D. I. A.; MELO, L. M.; FREITAS, V. J. F. |
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Afiliação: |
UNIVERSIDADE ESTADUAL DO CEARÁ; UNIVERSIDADE ESTADUAL DO CEARÁ; JEFERSON FERREIRA DA FONSECA, CNPC; UNIVERSIDADE ESTADUAL DO CEARÁ; UNIVERSIDADE ESTADUAL DO CEARÁ; UNIVERSIDADE ESTADUAL DO CEARÁ; UNIVERSIDADE ESTADUAL DO CEARÁ; INSTITUTE OF CYTOLOGY AND GENETICS; INSTITUTE OF CYTOLOGY AND GENETICS; UNIVERSIDADE ESTADUAL DO CEARÁ; UNIVERSIDADE ESTADUAL DO CEARÁ; UNIVERSIDADE ESTADUAL DO CEARÁ; UNIVERSIDADE ESTADUAL DO CEARÁ. |
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Título: |
Reproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Animal Reproduction, v. 11, n. 1, p. 37-43, Jan./Mar. 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fertility and capacity of transmitting the hG-CSF transgene to progeny, suggesting that the analyzed reproductive traits were not compromised by the presence of the transgene. MenosAbstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P > 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P > 0.05). No significant differences in embryo recovery rate (P > 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fer... Mostrar Tudo |
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Palavras-Chave: |
Transgenia. |
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Thesagro: |
Cabra; Caprino; Hormônio animal; Progesterona; Superovulação; Transferência de embrião. |
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Thesaurus Nal: |
Embryo transfer; Goats; Progesterone; Reproduction; Superovulation; Transgenesis. |
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Categoria do assunto: |
-- |
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Marc: |
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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