02896naa a2200421 a 450000100080000000500110000800800410001910000170006024501700007726000090024752016860025665000200194265000100196265000170197265000170198965000190200665000170202565000100204265000120205265000210206465000170208565000200210265000310212265300150215370000270216870000220219570000190221770000220223670000180225870000190227670000180229570000170231370000190233070000230234970000160237270000220238877300640241019855162025-01-10       2014    bl uuuu     u00u1 u    #d1 aMOURA, R. R.  aReproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene.h[electronic resource]  c2014  aAbstract: This study aimed to monitor estrous cycle parameters of a human granulocyte colony-stimulating factor (hG-CSF)-transgenic founder female goat and to perform superovulation and embryo recovery (surgical or transcervical method) for further transfer to recipients to quickly obtain offspring. Two experiments were performed using a transgenic (TF) and a non-transgenic (NTF) female. In experiment 1, three estrous cycles were monitored for the following parameters: estrus behavior, progesterone concentration and ovarian activity. In experiment 2, two superovulation/embryo recovery sessions were performed and the recovered embryos were transferred to previously prepared recipients. Data were compared by either t test or Fisher's exact test. The mean interval between natural estrus was 20.7 ± 0.6 and 19.7 ± 0.6 (P &gt 0.05) days for the TF and NTF, respectively. Progesterone concentrations and ovarian activity were normal and similar between goats. The ovulation rate was similar between TF and NTF (12.0 ± 1.4 vs. 18.0 ± 4.2 CL; P &gt 0.05). No significant differences in embryo recovery rate (P &gt 0.05) were observed between the surgical and transcervical methods for TF (69.2 vs. 72.7%) or NTF (100.0 vs. 86.7%). Sixteen embryos from the TF were transferred to recipients, and eight kids were born. Among these kids, the transgene was identified in three (two males and one female), resulting in a transgenesis rate of 37.5%. In summary, the TF is a true founder, since she proved fertility and capacity of transmitting the hG-CSF transgene to progeny, suggesting that the analyzed reproductive traits were not compromised by the presence of the transgene.  aEmbryo transfer  aGoats  aProgesterone  aReproduction  aSuperovulation  aTransgenesis  aCabra  aCaprino  aHormônio animal  aProgesterona  aSuperovulação  aTransferência de embrião  aTransgenia1 aSOUZA-FABJAN, J. M. G.1 aFONSECA, J. F. da1 aMELO, C. H. S.1 aSANCHEZ, D. J. D.1 aVIEIRA, M. P.1 aALMEIDA, T. M.1 aSEROVA, I. A.1 aSEROV, O. L.1 aPEREIRA, A. F.1 aTEIXEIRA, D. I. A.1 aMELO, L. M.1 aFREITAS, V. J. F.  tAnimal Reproductiongv. 11, n. 1, p. 37-43, Jan./Mar. 2014.