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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
13/02/2008 |
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Data da última atualização: |
24/07/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BENEDITO, V. A.; FARIA, L.; FREITAS-ÁSTUA, J.; FIGUEIRA, A. |
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Afiliação: |
Vagner Augusto Benedito, USP; Laura Faria, APTA; Juliana Freitas-Ástua, CNPMF; Antônio Figueira, USP. |
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Título: |
Genetic machinery for RNA silencing and defense against viruses in Citrus. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 991-996, 2007. |
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ISSN: |
1415-4757 |
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Idioma: |
Inglês |
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Conteúdo: |
RNA silencing mechanisms are conserved throughout eukaryotic evolution, possibly due to their importance in viral resistance and other aspects of cell biology. Here, we explored the Citrus EST (CitEST) database in search of sequences related to the most important known genes involved in RNA silencing. Transcripts strongly matching Argonaute (AGO), Dicer-like (DCL), Hua enhancer (HEN), and RNA-dependent RNA Polymerase (RdRP) were found in many of the citrus libraries. The reads were clustered and quantified. This shows that post-transcriptional gene silencing apparatus is active in citrus. It seems plausible that a better understanding of the players of RNA silencing in Citrus spp. and related genera may help create new tools to defeat the viral diseases that affect the citrus industry. Functional analyses of these citrus genes would enable the pursuit of this hypothesis. |
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Palavras-Chave: |
CitEST; PTGS. |
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Thesaurus Nal: |
gene silencing. |
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Categoria do assunto: |
-- |
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Marc: |
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022 $a1415-4757
100 1 $aBENEDITO, V. A.
245 $aGenetic machinery for RNA silencing and defense against viruses in Citrus.$h[electronic resource]
260 $c2007
520 $aRNA silencing mechanisms are conserved throughout eukaryotic evolution, possibly due to their importance in viral resistance and other aspects of cell biology. Here, we explored the Citrus EST (CitEST) database in search of sequences related to the most important known genes involved in RNA silencing. Transcripts strongly matching Argonaute (AGO), Dicer-like (DCL), Hua enhancer (HEN), and RNA-dependent RNA Polymerase (RdRP) were found in many of the citrus libraries. The reads were clustered and quantified. This shows that post-transcriptional gene silencing apparatus is active in citrus. It seems plausible that a better understanding of the players of RNA silencing in Citrus spp. and related genera may help create new tools to defeat the viral diseases that affect the citrus industry. Functional analyses of these citrus genes would enable the pursuit of this hypothesis.
650 $agene silencing
653 $aCitEST
653 $aPTGS
700 1 $aFARIA, L.
700 1 $aFREITAS-ÁSTUA, J.
700 1 $aFIGUEIRA, A.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 30, n. 3, p. 991-996, 2007.
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