01476naa a2200205 a 450000100080000000500110000800800410001902200140006010000200007424501020009426000090019652008880020565000190109365300110111265300090112370000140113270000230114670000170116977300840118616546582023-07-24 2007 bl uuuu u00u1 u #d a1415-47571 aBENEDITO, V. A. aGenetic machinery for RNA silencing and defense against viruses in Citrus.h[electronic resource] c2007 aRNA silencing mechanisms are conserved throughout eukaryotic evolution, possibly due to their importance in viral resistance and other aspects of cell biology. Here, we explored the Citrus EST (CitEST) database in search of sequences related to the most important known genes involved in RNA silencing. Transcripts strongly matching Argonaute (AGO), Dicer-like (DCL), Hua enhancer (HEN), and RNA-dependent RNA Polymerase (RdRP) were found in many of the citrus libraries. The reads were clustered and quantified. This shows that post-transcriptional gene silencing apparatus is active in citrus. It seems plausible that a better understanding of the players of RNA silencing in Citrus spp. and related genera may help create new tools to defeat the viral diseases that affect the citrus industry. Functional analyses of these citrus genes would enable the pursuit of this hypothesis. agene silencing aCitEST aPTGS1 aFARIA, L.1 aFREITAS-ÁSTUA, J.1 aFIGUEIRA, A. tGenetics and Molecular Biology, Ribeirão Pretogv. 30, n. 3, p. 991-996, 2007.