Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
27/10/2003 |
Data da última atualização: |
20/05/2024 |
Autoria: |
BLOOM, R. A.; LECHEVALIER, M. P.; TATE, R.L.-III. |
Título: |
Physiological, chemical, morphological, and plant infectivity characteristics of frankia isolates from Myrica pensylvanica: correlation to DNA restriction patterns. |
Ano de publicação: |
1989 |
Fonte/Imprenta: |
Applied and Environmental Microbiology, Washington, v. 55, n. 9, p. 2161-2166, 1989. |
Idioma: |
Inglês |
Conteúdo: |
The filter exclusion method was used to isolate Frankia strains from Myrica pensylvanica (bayberry) root nodules collected at diverse sites in New Jersey. A total of 16 isolates from five locations were cultured. The isolates were characterized by morphological, chemical, physiological, and plant infectivity criteria and compared with genomic DNA restriction pattern data, which were used to assign the isolates into gel groups (see accompanying paper). The isolates from M. pensylvanica evaluated in this study were characteristic of Frankia physiological group B strains and were indistinguishable on the basis of whole-cell wall chemistry and diaminopimelic acid isomer analysis. Distinct differences in the spectrum of utilized organic acids and carbohydrates were observed among the isolates and were the only phenotypic criteria by which the isolates could be separated and assigned into separate groups. In general, isolates within a restriction pattern gel group had identical utilization patterns, whereas intragroup isolates had different utilization patterns. Correlation of these phenotypic characteristics with the results of molecular analysis revealed an exclusive carbohydrate and organic acid utilization pattern for each gel group as established by restriction pattern analysis. |
Palavras-Chave: |
Characterization; Morphology; Myrica pensylvanica. |
Thesaurus Nal: |
Frankia; New Jersey; root nodules; strains. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02059naa a2200229 a 4500 001 1278560 005 2024-05-20 008 1989 bl uuuu u00u1 u #d 100 1 $aBLOOM, R. A. 245 $aPhysiological, chemical, morphological, and plant infectivity characteristics of frankia isolates from Myrica pensylvanica$bcorrelation to DNA restriction patterns.$h[electronic resource] 260 $c1989 520 $aThe filter exclusion method was used to isolate Frankia strains from Myrica pensylvanica (bayberry) root nodules collected at diverse sites in New Jersey. A total of 16 isolates from five locations were cultured. The isolates were characterized by morphological, chemical, physiological, and plant infectivity criteria and compared with genomic DNA restriction pattern data, which were used to assign the isolates into gel groups (see accompanying paper). The isolates from M. pensylvanica evaluated in this study were characteristic of Frankia physiological group B strains and were indistinguishable on the basis of whole-cell wall chemistry and diaminopimelic acid isomer analysis. Distinct differences in the spectrum of utilized organic acids and carbohydrates were observed among the isolates and were the only phenotypic criteria by which the isolates could be separated and assigned into separate groups. In general, isolates within a restriction pattern gel group had identical utilization patterns, whereas intragroup isolates had different utilization patterns. Correlation of these phenotypic characteristics with the results of molecular analysis revealed an exclusive carbohydrate and organic acid utilization pattern for each gel group as established by restriction pattern analysis. 650 $aFrankia 650 $aNew Jersey 650 $aroot nodules 650 $astrains 653 $aCharacterization 653 $aMorphology 653 $aMyrica pensylvanica 700 1 $aLECHEVALIER, M. P. 700 1 $aTATE, R.L.-III. 773 $tApplied and Environmental Microbiology, Washington$gv. 55, n. 9, p. 2161-2166, 1989.
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Registro original: |
Embrapa Florestas (CNPF) |
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