02059naa a2200229 a 450000100080000000500110000800800410001910000170006024501920007726000090026952013030027865000120158165000150159365000170160865000120162565300210163765300150165865300240167370000230169770000200172077300890174012785602024-05-20       1989    bl uuuu     u00u1 u    #d1 aBLOOM, R. A.  aPhysiological, chemical, morphological, and plant infectivity characteristics of frankia isolates from Myrica pensylvanicabcorrelation to DNA restriction patterns.h[electronic resource]  c1989  aThe filter exclusion method was used to isolate Frankia strains from Myrica pensylvanica (bayberry) root nodules collected at diverse sites in New Jersey. A total of 16 isolates from five locations were cultured. The isolates were characterized by morphological, chemical, physiological, and plant infectivity criteria and compared with genomic DNA restriction pattern data, which were used to assign the isolates into gel groups (see accompanying paper). The isolates from M. pensylvanica evaluated in this study were characteristic of Frankia physiological group B strains and were indistinguishable on the basis of whole-cell wall chemistry and diaminopimelic acid isomer analysis. Distinct differences in the spectrum of utilized organic acids and carbohydrates were observed among the isolates and were the only phenotypic criteria by which the isolates could be separated and assigned into separate groups. In general, isolates within a restriction pattern gel group had identical utilization patterns, whereas intragroup isolates had different utilization patterns. Correlation of these phenotypic characteristics with the results of molecular analysis revealed an exclusive carbohydrate and organic acid utilization pattern for each gel group as established by restriction pattern analysis.  aFrankia  aNew Jersey  aroot nodules  astrains  aCharacterization  aMorphology  aMyrica pensylvanica1 aLECHEVALIER, M. P.1 aTATE, R.L.-III.  tApplied and Environmental Microbiology, Washingtongv. 55, n. 9, p. 2161-2166, 1989.