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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
02/07/2025 |
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Data da última atualização: |
07/07/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENDONÇA, G. Z.; HAMINIUK, C. W. I.; PEDRO, A. C.; MARTINS, L. R. R.; MOLIN, F.; LARSEN, G. O. K.; HELM, C. V.; WENDLING, I.; MACIEL, G. M. |
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Afiliação: |
GUSTAVO ZAMBON MENDONÇA, UNIVERSIDADE TECNOLÓGICA FEDERAL DO PARANÁ; CHARLES WINDSON ISIDORO HAMINIUK, UNIVERSIDADE TECNOLÓGICA FEDERAL DO PARANÁ; ALESSANDRA CRISTINA PEDRO, UNIVERSIDADE TECNOLÓGICA FEDERAL DO PARANÁ; LUCIA REGINA ROCHA MARTINS, UNIVERSIDADE TECNOLÓGICA FEDERAL DO PARANÁ; FERNANDO MOLIN, UNIVERSIDADE TECNOLÓGICA FEDERAL DO PARANÁ; GABRIEL OALES KAMINSKI LARSEN, UNIVERSIDADE TECNOLÓGICA FEDERAL DO PARANÁ; CRISTIANE VIEIRA HELM, CNPF; IVAR WENDLING, CNPF; GISELLE MARIA MACIEL, UNIVERSIDADE TECNOLÓGICA FEDERAL DO PARANÁ. |
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Título: |
Extraction of bioactive compounds from different yerba mate genotypes and development of an antimicrobial gel. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Fitoterapia, v. 183, 106582, 2025. |
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DOI: |
https://doi.org/10.1016/j.fitote.2025.106582 |
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Idioma: |
Inglês |
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Conteúdo: |
Yerba mate (Ilex paraguariensis) is an essential source of bioactive compounds, such as polyphenols and methylxanthines, which provide health benefits and present potential antimicrobial properties. Nevertheless, the yerba mate genotypes and conditions to obtain plant extracts may influence the results of their biological potential. This paper describes the production of hydroethanolic extracts from two different genotypes of yerba mate (BRS and EC38) using a statistical experimental design. The total phenolic compounds (TPC), total flavones and flavonols (TFF), antioxidant activity, and antimicrobial potential were determined for each extract. Besides, some extracts were selected for chromatographic evaluation (LC-DAD and LC-QTOF-MS) and the development of an antimicrobial gel. The highest concentration of TPC was observed in BRS genotype (112 mg GAE/g DW) and the highest value of TFF was detected in EC38 genotype (19.45 QE/g DW). The highest values of antioxidant activities were 598.9 μmol TE/g DW for BRS and 753.7 μmol TE/g DW for EC38. A total of 16 phenolic compounds and flavonols were identified in yerba mate extracts by LC-QTOF-MS, and the EC38 genotype presented the highest number of compounds. The extracts from the BRS genotype had higher concentration of theobromine and caffeine, compared to the EC38 genotype. The yerba mate extracts could inhibit Escherichia coli, Staphylococcus aureus and Candida albicans, though at different levels. The yerba mate gels demonstrated equal and/or superior inhibitory capacity against E. coli and S. aureus compared to hand sanitizing gel produced only with ethanol (40 % and 70 %). MenosYerba mate (Ilex paraguariensis) is an essential source of bioactive compounds, such as polyphenols and methylxanthines, which provide health benefits and present potential antimicrobial properties. Nevertheless, the yerba mate genotypes and conditions to obtain plant extracts may influence the results of their biological potential. This paper describes the production of hydroethanolic extracts from two different genotypes of yerba mate (BRS and EC38) using a statistical experimental design. The total phenolic compounds (TPC), total flavones and flavonols (TFF), antioxidant activity, and antimicrobial potential were determined for each extract. Besides, some extracts were selected for chromatographic evaluation (LC-DAD and LC-QTOF-MS) and the development of an antimicrobial gel. The highest concentration of TPC was observed in BRS genotype (112 mg GAE/g DW) and the highest value of TFF was detected in EC38 genotype (19.45 QE/g DW). The highest values of antioxidant activities were 598.9 μmol TE/g DW for BRS and 753.7 μmol TE/g DW for EC38. A total of 16 phenolic compounds and flavonols were identified in yerba mate extracts by LC-QTOF-MS, and the EC38 genotype presented the highest number of compounds. The extracts from the BRS genotype had higher concentration of theobromine and caffeine, compared to the EC38 genotype. The yerba mate extracts could inhibit Escherichia coli, Staphylococcus aureus and Candida albicans, though at different levels. The yerba mate gels demonstra... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial activity; Atividade antimicrobiana; Bioactive compound; Composto bioativo; Erva mate; LC-MS/MS. |
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Thesagro: |
Gelatinização; Genótipo; Ilex Paraguariensis. |
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Thesaurus Nal: |
Bioactive compounds. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02686naa a2200349 a 4500
001 2176983
005 2025-07-07
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.fitote.2025.106582$2DOI
100 1 $aMENDONÇA, G. Z.
245 $aExtraction of bioactive compounds from different yerba mate genotypes and development of an antimicrobial gel.$h[electronic resource]
260 $c2025
520 $aYerba mate (Ilex paraguariensis) is an essential source of bioactive compounds, such as polyphenols and methylxanthines, which provide health benefits and present potential antimicrobial properties. Nevertheless, the yerba mate genotypes and conditions to obtain plant extracts may influence the results of their biological potential. This paper describes the production of hydroethanolic extracts from two different genotypes of yerba mate (BRS and EC38) using a statistical experimental design. The total phenolic compounds (TPC), total flavones and flavonols (TFF), antioxidant activity, and antimicrobial potential were determined for each extract. Besides, some extracts were selected for chromatographic evaluation (LC-DAD and LC-QTOF-MS) and the development of an antimicrobial gel. The highest concentration of TPC was observed in BRS genotype (112 mg GAE/g DW) and the highest value of TFF was detected in EC38 genotype (19.45 QE/g DW). The highest values of antioxidant activities were 598.9 μmol TE/g DW for BRS and 753.7 μmol TE/g DW for EC38. A total of 16 phenolic compounds and flavonols were identified in yerba mate extracts by LC-QTOF-MS, and the EC38 genotype presented the highest number of compounds. The extracts from the BRS genotype had higher concentration of theobromine and caffeine, compared to the EC38 genotype. The yerba mate extracts could inhibit Escherichia coli, Staphylococcus aureus and Candida albicans, though at different levels. The yerba mate gels demonstrated equal and/or superior inhibitory capacity against E. coli and S. aureus compared to hand sanitizing gel produced only with ethanol (40 % and 70 %).
650 $aBioactive compounds
650 $aGelatinização
650 $aGenótipo
650 $aIlex Paraguariensis
653 $aAntimicrobial activity
653 $aAtividade antimicrobiana
653 $aBioactive compound
653 $aComposto bioativo
653 $aErva mate
653 $aLC-MS/MS
700 1 $aHAMINIUK, C. W. I.
700 1 $aPEDRO, A. C.
700 1 $aMARTINS, L. R. R.
700 1 $aMOLIN, F.
700 1 $aLARSEN, G. O. K.
700 1 $aHELM, C. V.
700 1 $aWENDLING, I.
700 1 $aMACIEL, G. M.
773 $tFitoterapia$gv. 183, 106582, 2025.
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Registro original: |
Embrapa Florestas (CNPF) |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
05/06/2008 |
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Data da última atualização: |
03/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
MOURA, R. de C. de; MELO, N. F. de; SOUZA, M. J. de. |
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Afiliação: |
Rita de Cássia de Moura, UFPE; NATONIEL FRANKLIN DE MELO, CPATSA; Maria José de Souza, UFPE. |
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Título: |
High Levels of Chromosomal Differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae). |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 31, n. 2, p. 431-437, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Euchroma giganteawas karyotypically studied using conventional staining, C-banding, silver nitrate staining and ribosomal fluorescent in situ hybridization (rDNA FISH). Broad wide autosomal polymorphism and a complex sex determination system were found in this beetle. Karyotype complements ranging from 2n = 32, X1X2X3Y1Y2Y3 to 2n = 36,X1X2X3Y1Y2Y3 were detected in the sample analyzed. Punctiform suernumerary chromosomes were present in the different karyotypes. The karyotypic evolution of Brazilian E. giganteamay have taken two directions, reduction in the diploid number of 2n = 36 to 24 through centric fusions or 2n = 24 to 36 due to chromosomal fissions. In addition, pericentric inversions were also involved. The complex multiple sex mechanism of this species seems to be old and well established since it is found in specimens from different populations. Small pericentromeric blocks of constitutive heterochromatin were located on the autosomes and terminal blocks were also found on some small pairs. The sex chromosomes showed larger constitutive heterochromatin blocks. Silver nitrate staining during prophase I of meiosis showed labeling of the sex chromosome chain. However, the rDNA sites could only be precisely determined by FISH, which permitted the identification of these ribosomal sites on chromosomes X1 and X2 of this species. |
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Palavras-Chave: |
Cromossomo; DNAr; RON. |
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Thesagro: |
Coleóptero. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/162807/1/OPB1787.pdf
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Marc: |
LEADER 01919naa a2200193 a 4500
001 1162807
005 2018-09-03
008 2008 bl uuuu u00u1 u #d
100 1 $aMOURA, R. de C. de
245 $aHigh Levels of Chromosomal Differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae).
260 $c2008
520 $aEuchroma giganteawas karyotypically studied using conventional staining, C-banding, silver nitrate staining and ribosomal fluorescent in situ hybridization (rDNA FISH). Broad wide autosomal polymorphism and a complex sex determination system were found in this beetle. Karyotype complements ranging from 2n = 32, X1X2X3Y1Y2Y3 to 2n = 36,X1X2X3Y1Y2Y3 were detected in the sample analyzed. Punctiform suernumerary chromosomes were present in the different karyotypes. The karyotypic evolution of Brazilian E. giganteamay have taken two directions, reduction in the diploid number of 2n = 36 to 24 through centric fusions or 2n = 24 to 36 due to chromosomal fissions. In addition, pericentric inversions were also involved. The complex multiple sex mechanism of this species seems to be old and well established since it is found in specimens from different populations. Small pericentromeric blocks of constitutive heterochromatin were located on the autosomes and terminal blocks were also found on some small pairs. The sex chromosomes showed larger constitutive heterochromatin blocks. Silver nitrate staining during prophase I of meiosis showed labeling of the sex chromosome chain. However, the rDNA sites could only be precisely determined by FISH, which permitted the identification of these ribosomal sites on chromosomes X1 and X2 of this species.
650 $aColeóptero
653 $aCromossomo
653 $aDNAr
653 $aRON
700 1 $aMELO, N. F. de
700 1 $aSOUZA, M. J. de
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 31, n. 2, p. 431-437, 2008.
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