01918naa a2200193 a 450000100080000000500110000800800410001910000230006024501020008326000090018552013590019465000160155365300150156965300090158465300080159370000190160170000200162077300840164011628072018-09-03       2008    bl uuuu     u00u1 u    #d1 aMOURA, R. de C. de  aHigh Levels of Chromosomal Differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae)  c2008  aEuchroma giganteawas karyotypically studied using conventional staining, C-banding, silver nitrate staining and ribosomal fluorescent in situ hybridization (rDNA FISH). Broad wide autosomal polymorphism and a complex sex determination system were found in this beetle. Karyotype complements ranging from 2n = 32, X1X2X3Y1Y2Y3 to 2n = 36,X1X2X3Y1Y2Y3 were detected in the sample analyzed. Punctiform suernumerary chromosomes were present in the different karyotypes. The karyotypic evolution of Brazilian E. giganteamay have taken two directions, reduction in the diploid number of 2n = 36 to 24 through centric fusions or 2n = 24 to 36 due to chromosomal fissions. In addition, pericentric inversions were also involved. The complex multiple sex mechanism of this species seems to be old and well established since it is found in specimens from different populations. Small pericentromeric blocks of constitutive heterochromatin were located on the autosomes and terminal blocks were also found on some small pairs. The sex chromosomes showed larger constitutive heterochromatin blocks. Silver nitrate staining during prophase I of meiosis showed labeling of the sex chromosome chain. However, the rDNA sites could only be precisely determined by FISH, which permitted the identification of these ribosomal sites on chromosomes X1 and X2 of this species.  aColeóptero  aCromossomo  aDNAr  aRON1 aMELO, N. F. de1 aSOUZA, M. J. de  tGenetics and Molecular Biology, Ribeirão Pretogv. 31, n. 2, p. 431-437, 2008.