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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
26/09/2000 |
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Data da última atualização: |
08/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VALICENTE, F. H.; BARRETO, M. R.; VASCONCELOS, M. J. V. de; FIGUEIREDO, J. E. F. de; PAIVA, E. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS; MARIA JOSE VILACA DE VASCONCELOS, CNPMS. |
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Título: |
Identificação através de PCR dos genes Cryl de cepas de Bacillus thuringiensis Berliner eficientes contra a lagarta do cartucho, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae). |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Anais da Sociedade Entomológica do Brasil, Jaboticabal, v. 29, n. 1, p. 147-153, 2000. |
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Idioma: |
Português |
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Conteúdo: |
Fall armyworm, Spodoptera frugiperda (J.E. Smith), is the most important maize insect pest in Brazil and its damage can reduce yield up to 34%. The objective of this work was to utilize the PCR technique to identify Bacillus thuringiensis (B.t) strains that control S. frugiperda in maize crops. Biossays showed that 16 strains were very effective against S. frugiperda, 15 of them collected from soil samples and one (T09) obtained at the Institute Pasteur. The DNA of these strains were probed with cryl general primers and their proteins were analyzed by SDS-PAGE eletrophoresis. All strains present positive results for cryl genes. To further characterize these strains, specific cryl primers were empolyed. PCR technique showed that some strains harbour the same cryl genes. The only difference was the amplification of an unexpected fragment of approximately 160bp when a mixture of cry1B and cry1D specific primers was used. Analysis by phase contrast microscope showed that crystal proteins produced by these strains were all bipyramidal crystals. Also, eltrophoretic analysis of proteins by SDS-PAGE showed the same protein banding pattern for most of the strains. |
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Palavras-Chave: |
Cry1; Lagarta do cartucho. |
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Thesagro: |
Controle Biológico; Spodoptera Frugiperda. |
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Thesaurus Nal: |
biological control; Insecta. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/45185/1/Identificacao-atraves.pdf
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Marc: |
LEADER 02030naa a2200241 a 4500
001 1484159
005 2018-06-08
008 2000 bl uuuu u00u1 u #d
100 1 $aVALICENTE, F. H.
245 $aIdentificação através de PCR dos genes Cryl de cepas de Bacillus thuringiensis Berliner eficientes contra a lagarta do cartucho, Spodoptera frugiperda (J.E. Smith) (Lepidoptera$bNoctuidae).$h[electronic resource]
260 $c2000
520 $aFall armyworm, Spodoptera frugiperda (J.E. Smith), is the most important maize insect pest in Brazil and its damage can reduce yield up to 34%. The objective of this work was to utilize the PCR technique to identify Bacillus thuringiensis (B.t) strains that control S. frugiperda in maize crops. Biossays showed that 16 strains were very effective against S. frugiperda, 15 of them collected from soil samples and one (T09) obtained at the Institute Pasteur. The DNA of these strains were probed with cryl general primers and their proteins were analyzed by SDS-PAGE eletrophoresis. All strains present positive results for cryl genes. To further characterize these strains, specific cryl primers were empolyed. PCR technique showed that some strains harbour the same cryl genes. The only difference was the amplification of an unexpected fragment of approximately 160bp when a mixture of cry1B and cry1D specific primers was used. Analysis by phase contrast microscope showed that crystal proteins produced by these strains were all bipyramidal crystals. Also, eltrophoretic analysis of proteins by SDS-PAGE showed the same protein banding pattern for most of the strains.
650 $abiological control
650 $aInsecta
650 $aControle Biológico
650 $aSpodoptera Frugiperda
653 $aCry1
653 $aLagarta do cartucho
700 1 $aBARRETO, M. R.
700 1 $aVASCONCELOS, M. J. V. de
700 1 $aFIGUEIREDO, J. E. F. de
700 1 $aPAIVA, E.
773 $tAnais da Sociedade Entomológica do Brasil, Jaboticabal$gv. 29, n. 1, p. 147-153, 2000.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
13/05/2024 |
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Data da última atualização: |
13/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
DIAS, I. A.; HORTA, R. P.; MATOS, M.; HELM, C. V.; MAGALHAES, W. L. E.; LIMA, E. A. de; SILVA, B. J. G. da; MUNIZ, G. I. B. de; CADEMARTORI, P. H. G. de. |
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Afiliação: |
IVANA AMORIM DIAS, UNIVERSIDADE FEDERAL DO PARANÁ; RAYTA PAIM HORTA, UNIVERSIDADE FEDERAL DO PARANÁ; MAILSON MATOS, UNIVERSIDADE FEDERAL DO PARANÁ; CRISTIANE VIEIRA HELM, CNPF; WASHINGTON LUIZ ESTEVES MAGALHAES, CNPF; EDSON ALVES DE LIMA, CNPF; BRUNO JOSÉ GONÇALVES DA SILVA, UNIVERSIDADE FEDERAL DO PARANÁ; GRACIELA INES BOLZON DE MUNIZ, UNIVERSIDADE FEDERAL DO PARANÁ; PEDRO HENRIQUE GONZALEZ DE CADEMARTORI, UNIVERSIDADE FEDERAL DO PARANÁ. |
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Título: |
Exploring the antioxidant and antimicrobial properties of the water-soluble fraction derived from pyrolytic lignin separation in fast-pyrolysis bio-oil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Biomass Conversion and Biorefinery, p. 1-12, 2023. |
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DOI: |
http://dx.doi.org/10.1007/s13399-023-04561-7 |
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Idioma: |
Inglês |
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Notas: |
Online first. |
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Conteúdo: |
Improving key biomass functionalities necessitates effective liquid-liquid fractionation methods for heavy bio-oil. Here, fast-pyrolysis bio-oil derived from eucalypt wood was fractionated in cold water to separate insoluble and water-soluble fractions. We focus on the water-soluble fraction, a promising renewable source of chemicals with potential antioxidant and antimicrobial approaches. Fast-pyrolysis bio-oil was fractionated in three oil-to-water ratios, 1:100, 1:50, and 1:35, separating the pyrolytic lignin and producing water-soluble fractions as byproducts. The water-soluble fractions were analytically investigated through physicochemical properties, chemical composition by chromatography, phenolic content, antioxidant activity, antimicrobial activity, and their storage stability. Higher water content in the pyrolytic lignin separation resulted in higher yield and higher molecular weight compounds concentration in the water-soluble fractions, with the presence of highvalue- added chemicals, such as catechol, vanillin, and levoglucosan. Levoglucosan was the dominant chemical identified in the water-soluble fractions, notably for the 1:50 oil-to-water ratio. These remarkable chemicals and a high phenolic content contribute to both high antioxidant and antimicrobial activities. Despite the low concentration, all water-soluble fractions exhibited intense inhibition of Trametes versicolor and Gloeophyllum trabeum fungi. The fraction 1:50 completely inhibited the fungal activity. Both 1:100 and 1:50 fractions showed antibacterial activity for Escherichia coli and Staphylococcus aureus. Regardless of the oil-to-water ratio, water-soluble fractions exhibited a relative storage chemical stability under accelerated aging conditions for 43 days. Therefore, water-soluble fractions of fast-pyrolysis bio-oil derived from a simple method to separate pyrolytic lignin are functional materials with remarkable antioxidant and antimicrobial properties and, hence, upgrading bio-oil based on their functionalities. MenosImproving key biomass functionalities necessitates effective liquid-liquid fractionation methods for heavy bio-oil. Here, fast-pyrolysis bio-oil derived from eucalypt wood was fractionated in cold water to separate insoluble and water-soluble fractions. We focus on the water-soluble fraction, a promising renewable source of chemicals with potential antioxidant and antimicrobial approaches. Fast-pyrolysis bio-oil was fractionated in three oil-to-water ratios, 1:100, 1:50, and 1:35, separating the pyrolytic lignin and producing water-soluble fractions as byproducts. The water-soluble fractions were analytically investigated through physicochemical properties, chemical composition by chromatography, phenolic content, antioxidant activity, antimicrobial activity, and their storage stability. Higher water content in the pyrolytic lignin separation resulted in higher yield and higher molecular weight compounds concentration in the water-soluble fractions, with the presence of highvalue- added chemicals, such as catechol, vanillin, and levoglucosan. Levoglucosan was the dominant chemical identified in the water-soluble fractions, notably for the 1:50 oil-to-water ratio. These remarkable chemicals and a high phenolic content contribute to both high antioxidant and antimicrobial activities. Despite the low concentration, all water-soluble fractions exhibited intense inhibition of Trametes versicolor and Gloeophyllum trabeum fungi. The fraction 1:50 completely inhibited the fungal act... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial potential; Aqueous fraction; Levoglucosan; Storage stability. |
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Thesaurus NAL: |
Antioxidant activity; Phenolic compounds. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 03046naa a2200313 a 4500
001 2164243
005 2024-05-13
008 2023 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1007/s13399-023-04561-7$2DOI
100 1 $aDIAS, I. A.
245 $aExploring the antioxidant and antimicrobial properties of the water-soluble fraction derived from pyrolytic lignin separation in fast-pyrolysis bio-oil.$h[electronic resource]
260 $c2023
500 $aOnline first.
520 $aImproving key biomass functionalities necessitates effective liquid-liquid fractionation methods for heavy bio-oil. Here, fast-pyrolysis bio-oil derived from eucalypt wood was fractionated in cold water to separate insoluble and water-soluble fractions. We focus on the water-soluble fraction, a promising renewable source of chemicals with potential antioxidant and antimicrobial approaches. Fast-pyrolysis bio-oil was fractionated in three oil-to-water ratios, 1:100, 1:50, and 1:35, separating the pyrolytic lignin and producing water-soluble fractions as byproducts. The water-soluble fractions were analytically investigated through physicochemical properties, chemical composition by chromatography, phenolic content, antioxidant activity, antimicrobial activity, and their storage stability. Higher water content in the pyrolytic lignin separation resulted in higher yield and higher molecular weight compounds concentration in the water-soluble fractions, with the presence of highvalue- added chemicals, such as catechol, vanillin, and levoglucosan. Levoglucosan was the dominant chemical identified in the water-soluble fractions, notably for the 1:50 oil-to-water ratio. These remarkable chemicals and a high phenolic content contribute to both high antioxidant and antimicrobial activities. Despite the low concentration, all water-soluble fractions exhibited intense inhibition of Trametes versicolor and Gloeophyllum trabeum fungi. The fraction 1:50 completely inhibited the fungal activity. Both 1:100 and 1:50 fractions showed antibacterial activity for Escherichia coli and Staphylococcus aureus. Regardless of the oil-to-water ratio, water-soluble fractions exhibited a relative storage chemical stability under accelerated aging conditions for 43 days. Therefore, water-soluble fractions of fast-pyrolysis bio-oil derived from a simple method to separate pyrolytic lignin are functional materials with remarkable antioxidant and antimicrobial properties and, hence, upgrading bio-oil based on their functionalities.
650 $aAntioxidant activity
650 $aPhenolic compounds
653 $aAntimicrobial potential
653 $aAqueous fraction
653 $aLevoglucosan
653 $aStorage stability
700 1 $aHORTA, R. P.
700 1 $aMATOS, M.
700 1 $aHELM, C. V.
700 1 $aMAGALHAES, W. L. E.
700 1 $aLIMA, E. A. de
700 1 $aSILVA, B. J. G. da
700 1 $aMUNIZ, G. I. B. de
700 1 $aCADEMARTORI, P. H. G. de
773 $tBiomass Conversion and Biorefinery, p. 1-12, 2023.
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