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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
26/06/2012 |
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Data da última atualização: |
08/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KESKINTEPE, L.; SIMPLICIO, A. A.; BRACKETT, B. G. |
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Afiliação: |
Augusta Repro. Biology Associates, 812 Chafee Ave., United States; Aurino Alves Simplício, CNPC; Dept. of Physiol. and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, United States. |
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Título: |
Caprine blastocyst development after in vitro fertilization with spermatozoa frozen in different extenders. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Theriogenology, New York, v. 49, n. 7, p. 1265-1274, May, 1998. |
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DOI: |
10.1016/S0093-691X(98)00074-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The feasibility of using frozen-thawed semen in caprine IVF outside the breeding season was investigated. Electroejaculated spermatozoa from a Nubian buck were washed twice and then frozen in skim milk- or in egg yolk-based extenders. Goat oocytes were matured and inseminated by frozen-thawed spermatozoa selected by swim-up. In vitro fertilization was performed in a modified defined medium (mDM), altered experimentally, for 24 h. Embryos were cultured in 50 ?L of c-SOF+NEA for 9 d. The percentages of oocytes exposed to heparin-capacitated spermatozoa, (previously cryopreserved in skim milk- based extender) that cleaved, reached morula, blastocyst and expanded blastocyst stages were 82.8, 57.1, 35.7 and 30.0%, respectively. Without heparin treatment the rates for cleavage, morula, blastocyst and expanded blastocyst stages were 44.3, 31.4, 18.6 and 8.6%, respectively. Therefore, heparin treatment was included in sperm capacitation. Use of spermatozoa with BSA in the IVF medium yielded no cleavage. Although extenders containing 8 to 20% egg yolk enabled good sperm motility after cryopreservation, in vitro fertilizing ability was compromised under our conditions. By contrast, semen commercially processed in season in an egg yolk-based diluent remained effective for IVF. The highest proportion of blastocysts resulted from the use of spermatozoa diluted in a skim milk extender, heparin capacitation, and insemination in medium containing lamb serum. |
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Palavras-Chave: |
Animal embryos; Blastocisto; Extenders; Ovary; Sperm cryopreservation; Sperm preservation. |
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Thesagro: |
Caprino; Criopreservação; Espermatozóide; Inseminação artificial; Reprodução animal; Sêmen. |
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Thesaurus Nal: |
Animal breeding; Blastocystis; Goats; In vitro fertilization; Reproduction. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02532naa a2200361 a 4500
001 1927208
005 2017-09-08
008 1998 bl uuuu u00u1 u #d
024 7 $a10.1016/S0093-691X(98)00074-0$2DOI
100 1 $aKESKINTEPE, L.
245 $aCaprine blastocyst development after in vitro fertilization with spermatozoa frozen in different extenders.$h[electronic resource]
260 $c1998
520 $aAbstract: The feasibility of using frozen-thawed semen in caprine IVF outside the breeding season was investigated. Electroejaculated spermatozoa from a Nubian buck were washed twice and then frozen in skim milk- or in egg yolk-based extenders. Goat oocytes were matured and inseminated by frozen-thawed spermatozoa selected by swim-up. In vitro fertilization was performed in a modified defined medium (mDM), altered experimentally, for 24 h. Embryos were cultured in 50 ?L of c-SOF+NEA for 9 d. The percentages of oocytes exposed to heparin-capacitated spermatozoa, (previously cryopreserved in skim milk- based extender) that cleaved, reached morula, blastocyst and expanded blastocyst stages were 82.8, 57.1, 35.7 and 30.0%, respectively. Without heparin treatment the rates for cleavage, morula, blastocyst and expanded blastocyst stages were 44.3, 31.4, 18.6 and 8.6%, respectively. Therefore, heparin treatment was included in sperm capacitation. Use of spermatozoa with BSA in the IVF medium yielded no cleavage. Although extenders containing 8 to 20% egg yolk enabled good sperm motility after cryopreservation, in vitro fertilizing ability was compromised under our conditions. By contrast, semen commercially processed in season in an egg yolk-based diluent remained effective for IVF. The highest proportion of blastocysts resulted from the use of spermatozoa diluted in a skim milk extender, heparin capacitation, and insemination in medium containing lamb serum.
650 $aAnimal breeding
650 $aBlastocystis
650 $aGoats
650 $aIn vitro fertilization
650 $aReproduction
650 $aCaprino
650 $aCriopreservação
650 $aEspermatozóide
650 $aInseminação artificial
650 $aReprodução animal
650 $aSêmen
653 $aAnimal embryos
653 $aBlastocisto
653 $aExtenders
653 $aOvary
653 $aSperm cryopreservation
653 $aSperm preservation
700 1 $aSIMPLICIO, A. A.
700 1 $aBRACKETT, B. G.
773 $tTheriogenology, New York$gv. 49, n. 7, p. 1265-1274, May, 1998.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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