02532naa a2200361 a 450000100080000000500110000800800410001902400390006010000190009924501350011826000090025352014810026265000200174365000170176365000100178065000270179065000170181765000120183465000220184665000200186865000290188865000240191765000110194165300190195265300160197165300140198765300100200165300270201165300230203870000210206170000200208277300680210219272082017-09-08       1998    bl uuuu     u00u1 u    #d7 a10.1016/S0093-691X(98)00074-02DOI1 aKESKINTEPE, L.  aCaprine blastocyst development after in vitro fertilization with spermatozoa frozen in different extenders.h[electronic resource]  c1998  aAbstract: The feasibility of using frozen-thawed semen in caprine IVF outside the breeding season was investigated. Electroejaculated spermatozoa from a Nubian buck were washed twice and then frozen in skim milk- or in egg yolk-based extenders. Goat oocytes were matured and inseminated by frozen-thawed spermatozoa selected by swim-up. In vitro fertilization was performed in a modified defined medium (mDM), altered experimentally, for 24 h. Embryos were cultured in 50 ?L of c-SOF+NEA for 9 d. The percentages of oocytes exposed to heparin-capacitated spermatozoa, (previously cryopreserved in skim milk- based extender) that cleaved, reached morula, blastocyst and expanded blastocyst stages were 82.8, 57.1, 35.7 and 30.0%, respectively. Without heparin treatment the rates for cleavage, morula, blastocyst and expanded blastocyst stages were 44.3, 31.4, 18.6 and 8.6%, respectively. Therefore, heparin treatment was included in sperm capacitation. Use of spermatozoa with BSA in the IVF medium yielded no cleavage. Although extenders containing 8 to 20% egg yolk enabled good sperm motility after cryopreservation, in vitro fertilizing ability was compromised under our conditions. By contrast, semen commercially processed in season in an egg yolk-based diluent remained effective for IVF. The highest proportion of blastocysts resulted from the use of spermatozoa diluted in a skim milk extender, heparin capacitation, and insemination in medium containing lamb serum.  aAnimal breeding  aBlastocystis  aGoats  aIn vitro fertilization  aReproduction  aCaprino  aCriopreservação  aEspermatozóide  aInseminação artificial  aReprodução animal  aSêmen  aAnimal embryos  aBlastocisto  aExtenders  aOvary  aSperm cryopreservation  aSperm preservation1 aSIMPLICIO, A. A.1 aBRACKETT, B. G.  tTheriogenology, New Yorkgv. 49, n. 7, p. 1265-1274, May, 1998.