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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
25/01/1996 |
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Data da última atualização: |
13/06/2023 |
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Autoria: |
GEORGE, S.; SRIVASTAVA, R. K. |
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Título: |
Activity of phosphatases, isomerase and dehydrogenase in ram semen. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Journal of Veterinary and Animal Science, v. 21, n. 2, p. 24-29, 1990. |
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Idioma: |
Inglês |
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Conteúdo: |
Experiments were conducted in freshly collected semen samples having a +5 motility of healthy native (Muzaffar nagri) and cross-bred (Muzaffar nagri Dorsett) rams or age 3-3 1/2 years. Activity of the enzymes, alkaline phosphatase, acid phosphatase, glucose 6-phosphatase, phosphogiucoisomarase and sorbitol dehydrogenase were assayed in seminal plasma, sperm pack, ultrasonicate extract and ultrasonicate pack. The ultrasonicate extract was prepared by sonicating the semen pellet suspended in a suitable buffer (PBS/Tris) at 20 KCS using a vibronic ultrasonicator. The seminal plasma alkaline phosphatase activity was signifiicantly higher (P < 0.01) in both the breeda as compared to the other fractions. But a significant difference (P<0.01)in the seminal plasma activity was observed between the breeds, being higher in cross-bred. The soluble form of the enzyme was 65 to 70% and particulate bound was 30 to 35% in both the breeds. Acid phosphatase activity was higher in ultrasonicate extract compared to seminal plasma sperm pack and uirasonicate pack in both the breeds. Significant difference (P < 0.05) was observed in the seminal plasma activity, being higher in native breed. The soluble form of the enzyme was 80 to 82% and 18 to 20% was particulate bound. Glucose 6-phosphatase activity did not differ significantly between breeds. The soluble form of the enzyma was 45% and particulate bound was 55% it native breed and vice versa in cross-bred. Phosphoglucoisomerase activity in the seminal plasma was significantly lower (P <0.01) as compered to the sperm pack in both the breeds. About 92 to 95% of the enzyme was in the soluble form and 5 to 8% in particulate bound. Sorbitol dehydrogenase activity appearod to be low in seminal plasma as compared to sperm pack in both the breeds. The soluble form of the enzyma was 74% and 82% and particulate bound was 26% and 18% in native and cross-bred respectively. MenosExperiments were conducted in freshly collected semen samples having a +5 motility of healthy native (Muzaffar nagri) and cross-bred (Muzaffar nagri Dorsett) rams or age 3-3 1/2 years. Activity of the enzymes, alkaline phosphatase, acid phosphatase, glucose 6-phosphatase, phosphogiucoisomarase and sorbitol dehydrogenase were assayed in seminal plasma, sperm pack, ultrasonicate extract and ultrasonicate pack. The ultrasonicate extract was prepared by sonicating the semen pellet suspended in a suitable buffer (PBS/Tris) at 20 KCS using a vibronic ultrasonicator. The seminal plasma alkaline phosphatase activity was signifiicantly higher (P < 0.01) in both the breeda as compared to the other fractions. But a significant difference (P<0.01)in the seminal plasma activity was observed between the breeds, being higher in cross-bred. The soluble form of the enzyme was 65 to 70% and particulate bound was 30 to 35% in both the breeds. Acid phosphatase activity was higher in ultrasonicate extract compared to seminal plasma sperm pack and uirasonicate pack in both the breeds. Significant difference (P < 0.05) was observed in the seminal plasma activity, being higher in native breed. The soluble form of the enzyme was 80 to 82% and 18 to 20% was particulate bound. Glucose 6-phosphatase activity did not differ significantly between breeds. The soluble form of the enzyma was 45% and particulate bound was 55% it native breed and vice versa in cross-bred. Phosphoglucoisomerase activity in the... Mostrar Tudo |
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Palavras-Chave: |
Dehidrogenase; Fosfatases; Glucose 6 phosphate dehydrogenase. |
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Thesagro: |
Bioquímica; Ovino; Reprodução; Sêmen. |
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Thesaurus Nal: |
Acid phosphatase; Alkaline phosphatase; Enzymes; Isomerases; Rams; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02727naa a2200301 a 4500
001 1523539
005 2023-06-13
008 1990 bl uuuu u00u1 u #d
100 1 $aGEORGE, S.
245 $aActivity of phosphatases, isomerase and dehydrogenase in ram semen.
260 $c1990
520 $aExperiments were conducted in freshly collected semen samples having a +5 motility of healthy native (Muzaffar nagri) and cross-bred (Muzaffar nagri Dorsett) rams or age 3-3 1/2 years. Activity of the enzymes, alkaline phosphatase, acid phosphatase, glucose 6-phosphatase, phosphogiucoisomarase and sorbitol dehydrogenase were assayed in seminal plasma, sperm pack, ultrasonicate extract and ultrasonicate pack. The ultrasonicate extract was prepared by sonicating the semen pellet suspended in a suitable buffer (PBS/Tris) at 20 KCS using a vibronic ultrasonicator. The seminal plasma alkaline phosphatase activity was signifiicantly higher (P < 0.01) in both the breeda as compared to the other fractions. But a significant difference (P<0.01)in the seminal plasma activity was observed between the breeds, being higher in cross-bred. The soluble form of the enzyme was 65 to 70% and particulate bound was 30 to 35% in both the breeds. Acid phosphatase activity was higher in ultrasonicate extract compared to seminal plasma sperm pack and uirasonicate pack in both the breeds. Significant difference (P < 0.05) was observed in the seminal plasma activity, being higher in native breed. The soluble form of the enzyme was 80 to 82% and 18 to 20% was particulate bound. Glucose 6-phosphatase activity did not differ significantly between breeds. The soluble form of the enzyma was 45% and particulate bound was 55% it native breed and vice versa in cross-bred. Phosphoglucoisomerase activity in the seminal plasma was significantly lower (P <0.01) as compered to the sperm pack in both the breeds. About 92 to 95% of the enzyme was in the soluble form and 5 to 8% in particulate bound. Sorbitol dehydrogenase activity appearod to be low in seminal plasma as compared to sperm pack in both the breeds. The soluble form of the enzyma was 74% and 82% and particulate bound was 26% and 18% in native and cross-bred respectively.
650 $aAcid phosphatase
650 $aAlkaline phosphatase
650 $aEnzymes
650 $aIsomerases
650 $aRams
650 $aReproduction
650 $aSheep
650 $aBioquímica
650 $aOvino
650 $aReprodução
650 $aSêmen
653 $aDehidrogenase
653 $aFosfatases
653 $aGlucose 6 phosphate dehydrogenase
700 1 $aSRIVASTAVA, R. K.
773 $tJournal of Veterinary and Animal Science$gv. 21, n. 2, p. 24-29, 1990.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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