02727naa a2200301 a 450000100080000000500110000800800410001910000150006024500720007526000090014752019380015665000210209465000250211565000120214065000150215265000090216765000170217665000100219365000160220365000100221965000170222965000110224665300180225765300150227565300380229070000220232877300750235015235392023-06-13       1990    bl uuuu     u00u1 u    #d1 aGEORGE, S.  aActivity of phosphatases, isomerase and dehydrogenase in ram semen.  c1990  aExperiments were conducted in freshly collected semen samples having a +5 motility of healthy native (Muzaffar nagri) and cross-bred (Muzaffar nagri Dorsett) rams or age 3-3 1/2 years. Activity of the enzymes, alkaline phosphatase, acid phosphatase, glucose 6-phosphatase, phosphogiucoisomarase and sorbitol dehydrogenase were assayed in seminal plasma, sperm pack, ultrasonicate extract and ultrasonicate pack. The ultrasonicate extract was prepared by sonicating the semen pellet suspended in a suitable buffer (PBS/Tris) at 20 KCS using a vibronic ultrasonicator. The seminal plasma alkaline phosphatase activity was signifiicantly higher (P &lt 0.01) in both the breeda as compared to the other fractions. But a significant difference (P&lt0.01)in the seminal plasma activity was observed between the breeds, being higher in cross-bred. The soluble form of the enzyme was 65 to 70% and particulate bound was 30 to 35% in both the breeds. Acid phosphatase activity was higher in ultrasonicate extract compared to seminal plasma sperm pack and uirasonicate pack in both the breeds. Significant difference (P &lt 0.05) was observed in the seminal plasma activity, being higher in native breed. The soluble form of the enzyme was 80 to 82% and 18 to 20% was particulate bound. Glucose 6-phosphatase activity did not differ significantly between breeds. The soluble form of the enzyma was 45% and particulate bound was 55% it native breed and vice versa in cross-bred. Phosphoglucoisomerase activity in the seminal plasma was significantly lower (P &lt0.01) as compered to the sperm pack in both the breeds. About 92 to 95% of the enzyme was in the soluble form and 5 to 8% in particulate bound. Sorbitol dehydrogenase activity appearod to be low in seminal plasma as compared to sperm pack in both the breeds. The soluble form of the enzyma was 74% and 82% and particulate bound was 26% and 18% in native and cross-bred respectively.  aAcid phosphatase  aAlkaline phosphatase  aEnzymes  aIsomerases  aRams  aReproduction  aSheep  aBioquímica  aOvino  aReprodução  aSêmen  aDehidrogenase  aFosfatases  aGlucose 6 phosphate dehydrogenase1 aSRIVASTAVA, R. K.  tJournal of Veterinary and Animal Sciencegv. 21, n. 2, p. 24-29, 1990.