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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
29/03/2023 |
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Autoria: |
FUKUHARA, R.; NISHIKAWA, Y. |
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Título: |
Effects of PH, sperm concentration, washing and substrate concentration on respiration and motility of goat spermatozoa. |
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Ano de publicação: |
1973 |
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Fonte/Imprenta: |
Japanese Journal Zootchnie Science, v. 44, n. 5, p. 266-270, 1973. |
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DOI: |
https://doi.org/10.2508/chikusan.44.266 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The effects of pH, sperm concentration, washing and substrate concentration and motility of ejaculated goat spermatozoa were studied at 37?,using? the manometric method of Warburg. The results obtained are as follows. 1. The optimum pH was 7.2 to 7.5 for sperm respiration, and 7.0 to 7.2 for sperm motility. 2. Sperm concentrations ranging from 0.5 to 4.0x108 per ml of sperm suspension gave no effect on the respiration. 3. To study the effect of an exogenous substrate on the respiration of goat spermatozoa, it is necessary to wash the ejaculated sperm sample twice at least. 4. The optimum concentration of exogeneous substrate was 10-2 to 5x10-2 M for metabolic studies of goat spermatozoa. Table 1. Composition of buffered diluents; Table 2. Effect of pH of buffer on respiration and motility of goat spermatozoa; Table 3. Effect of sperm density on respiration and motility of goat spermatozoa (Mean of six measurements). Table 4. Effect of washing on respiration and motility of goat spermatozoa (Mean of three measurements); Fig. 2. Effect of substrate concentration on respiration and motility of goat spermatozoa (Mean of two measurements) |
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Thesagro: |
Bioquímica; Caprino; Reprodução; Sêmen. |
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Thesaurus Nal: |
Goats; Reproduction; Sperm motility. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01870naa a2200229 a 4500
001 1521336
005 2023-03-29
008 1973 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.2508/chikusan.44.266$2DOI
100 1 $aFUKUHARA, R.
245 $aEffects of PH, sperm concentration, washing and substrate concentration on respiration and motility of goat spermatozoa.$h[electronic resource]
260 $c1973
520 $aAbstract: The effects of pH, sperm concentration, washing and substrate concentration and motility of ejaculated goat spermatozoa were studied at 37?,using? the manometric method of Warburg. The results obtained are as follows. 1. The optimum pH was 7.2 to 7.5 for sperm respiration, and 7.0 to 7.2 for sperm motility. 2. Sperm concentrations ranging from 0.5 to 4.0x108 per ml of sperm suspension gave no effect on the respiration. 3. To study the effect of an exogenous substrate on the respiration of goat spermatozoa, it is necessary to wash the ejaculated sperm sample twice at least. 4. The optimum concentration of exogeneous substrate was 10-2 to 5x10-2 M for metabolic studies of goat spermatozoa. Table 1. Composition of buffered diluents; Table 2. Effect of pH of buffer on respiration and motility of goat spermatozoa; Table 3. Effect of sperm density on respiration and motility of goat spermatozoa (Mean of six measurements). Table 4. Effect of washing on respiration and motility of goat spermatozoa (Mean of three measurements); Fig. 2. Effect of substrate concentration on respiration and motility of goat spermatozoa (Mean of two measurements)
650 $aGoats
650 $aReproduction
650 $aSperm motility
650 $aBioquímica
650 $aCaprino
650 $aReprodução
650 $aSêmen
700 1 $aNISHIKAWA, Y.
773 $tJapanese Journal Zootchnie Science$gv. 44, n. 5, p. 266-270, 1973.
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| 1. |  | NUNES, C. da S.; VELASQUEZ, P. A. T.; CARILHO, E. N. V. M.; SOUZA, G. B. de; NOGUEIRA, A. R. de A.; OLIVEIRA, S. G.; BERCHIELLI, T. T. Material alternativo para confecção de filtros empregados na metodologia "Nylon bag" para a determinação de fibra. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 42., 2005, Goiânia. A Produção animal e o foco no agronegócio. Anais... Goiânia: SBZ, 2005. 5 p.| Tipo: Artigo em Anais de Congresso |
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