01870naa a2200229 a 450000100080000000500110000800800410001902400490006010000170010924501480012626000090027452011660028365000100144965000170145965000190147665000160149565000120151165000170152365000110154070000180155177300710156915213362023-03-29       1973    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.2508/chikusan.44.2662DOI1 aFUKUHARA, R.  aEffects of PH, sperm concentration, washing and substrate concentration on respiration and motility of goat spermatozoa.h[electronic resource]  c1973  aAbstract: The effects of pH, sperm concentration, washing and substrate concentration and motility of ejaculated goat spermatozoa were studied at 37?,using? the manometric method of Warburg. The results obtained are as follows. 1. The optimum pH was 7.2 to 7.5 for sperm respiration, and 7.0 to 7.2 for sperm motility. 2. Sperm concentrations ranging from 0.5 to 4.0x108 per ml of sperm suspension gave no effect on the respiration. 3. To study the effect of an exogenous substrate on the respiration of goat spermatozoa, it is necessary to wash the ejaculated sperm sample twice at least. 4. The optimum concentration of exogeneous substrate was 10-2 to 5x10-2 M for metabolic studies of goat spermatozoa. Table 1. Composition of buffered diluents; Table 2. Effect of pH of buffer on respiration and motility of goat spermatozoa; Table 3. Effect of sperm density on respiration and motility of goat spermatozoa (Mean of six measurements). Table 4. Effect of washing on respiration and motility of goat spermatozoa (Mean of three measurements); Fig. 2. Effect of substrate concentration on respiration and motility of goat spermatozoa (Mean of two measurements)  aGoats  aReproduction  aSperm motility  aBioquímica  aCaprino  aReprodução  aSêmen1 aNISHIKAWA, Y.  tJapanese Journal Zootchnie Sciencegv. 44, n. 5, p. 266-270, 1973.