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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
26/01/2015 |
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Data da última atualização: |
26/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HALLWASS, M.; OLIVEIRA, A. S. de; DIANESE, E. de C.; LOHUIS, D.; BOITEUX, L. S.; NAGATA, A. K. I.; RESENDE, R. O.; KORMELINK, R. |
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Afiliação: |
MARIANA HALLWASS, LABORATORY OF VIROLOGY, DEPATMENT OF PLANT SCIENCES, WAGENINGEN UNIVERSITY.; ATHOS SILVA DE OLIVEIRA, INSTITUTODE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGIA CELUALAR, UNIVERSIDADE DE BRASILIA.; ERICO DE CAMPOS DIANESE, INSTITUTO DE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGIA CELULAR, UNIVERSIDADE DE BRASÍLIA.; DICK LOHUIS, INSTITUTO DE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGIA CELULAR, UNIVERSIDADE DE BRASÍLIA.; LEONARDO SILVA BOITEUX, CNPH; ALICE KAZUKO INOUE NAGATA, CNPH; RENATO O. RESENDE, INSTITUTO DE CIENCIAS BIOLÓGICAS, DEPARTAMENTO DE BIOLOGIA CELULAR, UNIVERSIDADE DE BRASÍLIA.; RICHARD KORMELINK, LABORATORY OF VIROLOGY, DEPARTMENT OF PLANT SCIENCES, WAGENINGEN UNIVERSITY. |
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Título: |
The tomato spotted wilt virus cell-to-cell movement protein (NSM) triggers a hypersensitive response in Sw-5-containing resistant tomato lines and in Nicotiana benthamiana transformed with the functional Sw-5b resistance gene copy. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Molecular Plant Pathology, v. 15, n. 9, p. 871-880, 2014. |
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ISSN: |
1364-3703 |
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DOI: |
10.1111/mpp.12144 |
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Idioma: |
Inglês |
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Conteúdo: |
Although the Sw-5 gene cluster has been cloned, and Sw-5b has been identified as the functional gene copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr) determinant has not been identified to date. Nicotiana tabacum ?SR1? plants transformed with a copy of the Sw-5b gene are immune without producing a clear visual response on challenge with TSWV, whereas it is shown here that N. benthamiana transformed with Sw-5b gives a rapid and conspicuous hypersensitive response (HR). Using these plants, from all structural and non-structural TSWV proteins tested, the TSWV cell-to-cell movement protein (NSM) was confirmed as the Avr determinant using a Potato virus X (PVX) replicon or a non-replicative pEAQ-HT expression vector system. HR was induced in Sw-5b-transgenic N. benthamiana as well as in resistant near-isogenic tomato lines after agroinfiltration with a functional cell-to-cell movement protein (NSM) from a resistance-inducing (RI) TSWV strain (BR-01), but not with NSM from a Sw-5 resistance-breaking (RB) strain (GRAU).This is the first biological demonstration that Sw-5 -mediated resist-ance is triggered by the TSWV NSM cell-to-cell movement protein. |
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Palavras-Chave: |
Avirulence; Cell-to-cell movement; Resistance gene; Sw-5; TSWV. |
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Thesaurus Nal: |
Tospovirus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02186naa a2200301 a 4500
001 2006718
005 2015-01-26
008 2014 bl uuuu u00u1 u #d
022 $a1364-3703
024 7 $a10.1111/mpp.12144$2DOI
100 1 $aHALLWASS, M.
245 $aThe tomato spotted wilt virus cell-to-cell movement protein (NSM) triggers a hypersensitive response in Sw-5-containing resistant tomato lines and in Nicotiana benthamiana transformed with the functional Sw-5b resistance gene copy.$h[electronic resource]
260 $c2014
520 $aAlthough the Sw-5 gene cluster has been cloned, and Sw-5b has been identified as the functional gene copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr) determinant has not been identified to date. Nicotiana tabacum ?SR1? plants transformed with a copy of the Sw-5b gene are immune without producing a clear visual response on challenge with TSWV, whereas it is shown here that N. benthamiana transformed with Sw-5b gives a rapid and conspicuous hypersensitive response (HR). Using these plants, from all structural and non-structural TSWV proteins tested, the TSWV cell-to-cell movement protein (NSM) was confirmed as the Avr determinant using a Potato virus X (PVX) replicon or a non-replicative pEAQ-HT expression vector system. HR was induced in Sw-5b-transgenic N. benthamiana as well as in resistant near-isogenic tomato lines after agroinfiltration with a functional cell-to-cell movement protein (NSM) from a resistance-inducing (RI) TSWV strain (BR-01), but not with NSM from a Sw-5 resistance-breaking (RB) strain (GRAU).This is the first biological demonstration that Sw-5 -mediated resist-ance is triggered by the TSWV NSM cell-to-cell movement protein.
650 $aTospovirus
653 $aAvirulence
653 $aCell-to-cell movement
653 $aResistance gene
653 $aSw-5
653 $aTSWV
700 1 $aOLIVEIRA, A. S. de
700 1 $aDIANESE, E. de C.
700 1 $aLOHUIS, D.
700 1 $aBOITEUX, L. S.
700 1 $aNAGATA, A. K. I.
700 1 $aRESENDE, R. O.
700 1 $aKORMELINK, R.
773 $tMolecular Plant Pathology$gv. 15, n. 9, p. 871-880, 2014.
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| 2. |  | SUGAI, L. S. M.; COSTA-PEREIRA, R.; QUINTEIRO, J. M. O.; TORRECILHA, S.; ERIKSSON, A.; NUNES, A. P.; KEUROGHLIAN, A.; ARAUJO, A. C.; PARANHOS FILHO, A. C.; DESBIEZ, A. L. J.; POTT, A.; SANTOS, C. F.; FERREIRA, C. M. M.; RIBEIRO, D. B.; EATON, D. P.; SCREMIN-DIAS, E.; CORRÊA, EL. C.; BURKHARDT, E.; FISCHER, E.; AYRES, F. M.; MARTINS, F. I.; ALVES, F. M.; SEVERO-NETO, F.; SOUZA, F. L.; PAULA, G. A. DE.; SOUZA, G. N. de; SEIXAS, G. H. F.; DALPONTI, G.; BARROS, G. M. de; GRACIOLLI, G.; GIMÊNES JUNIOR, H.; ILHA, I. M. N.; SUGAI, J. L. M. M.; LONGO, J. M.; SABINO, J.; CORDEIRO, L. M.; NASCIMENTO, L. N.; TENCATT, L. F. C.; BUENO, M. L.; RODRIGUES, M. E.; BENITES, M.; SILVA, M.; GODOI, M. N.; SILVEIRA, M.; DELATORRE, M.; RIBEIRO, M. C.; GUEDES, N. M. R.; CUNHA, N. L. da; FROEHLICH, O.; MEDICI, P.; MEDINA JUNIOR, P. B.; SOUZA, P. R. de; FERREIRA, R. P.; LOURIVAL, R. F. F.; GAMARRA, R. M.; BORGHEZAN, R.; LAPS, R. R.; ROCHA, S. B. da; DULEBA, S.; MAMEDE, S.; ESCAPINATI, S. C.; SINANI, T. R. F.; FERREIRA, V. L.; TOMAS, W. M.; SÚAREZ, Y. R.; ROQUE, F. de O. Incorporating biodiversity expert knowledge in landscape conservation planning: a case study involving the Pantanal. In: SIMPÓSIO DE GEOTECNOLOGIAS NO PANTANAL, 5., 2014, Campo Grande, MS. Anais... São José dos Campos: INPE, 2014. p. 544-553. 1 CD-ROM. Geopantanal 2014.| Tipo: Artigo em Anais de Congresso |
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