02186naa a2200301 a 450000100080000000500110000800800410001902200140006002400270007410000170010124502590011826000090037752012050038665000150159165300150160665300260162165300200164765300090166765300090167670000230168570000220170870000150173070000190174570000210176470000190178570000180180477300620182220067182015-01-26       2014    bl uuuu     u00u1 u    #d  a1364-37037 a10.1111/mpp.121442DOI1 aHALLWASS, M.  aThe tomato spotted wilt virus cell-to-cell movement protein (NSM) triggers a hypersensitive response in Sw-5-containing resistant tomato lines and in Nicotiana benthamiana transformed with the functional Sw-5b resistance gene copy.h[electronic resource]  c2014  aAlthough the Sw-5 gene cluster has been cloned, and Sw-5b has been identified as the functional gene copy that confers resistance to Tomato spotted wilt virus (TSWV), its avirulence (Avr) determinant has not been identified to date. Nicotiana tabacum ?SR1? plants transformed with a copy of the Sw-5b gene are immune without producing a clear visual response on challenge with TSWV, whereas it is shown here that N. benthamiana transformed with Sw-5b gives a rapid and conspicuous hypersensitive response (HR). Using these plants, from all structural and non-structural TSWV proteins tested, the TSWV cell-to-cell movement protein (NSM) was confirmed as the Avr determinant using a Potato virus X (PVX) replicon or a non-replicative pEAQ-HT expression vector system. HR was induced in Sw-5b-transgenic N. benthamiana as well as in resistant near-isogenic tomato lines after agroinfiltration with a functional cell-to-cell movement protein (NSM) from a resistance-inducing (RI) TSWV strain (BR-01), but not with NSM from a Sw-5 resistance-breaking (RB) strain (GRAU).This is the first biological demonstration that Sw-5 -mediated resist-ance is triggered by the TSWV NSM cell-to-cell movement protein.  aTospovirus  aAvirulence  aCell-to-cell movement  aResistance gene  aSw-5  aTSWV1 aOLIVEIRA, A. S. de1 aDIANESE, E. de C.1 aLOHUIS, D.1 aBOITEUX, L. S.1 aNAGATA, A. K. I.1 aRESENDE, R. O.1 aKORMELINK, R.  tMolecular Plant Pathologygv. 15, n. 9, p. 871-880, 2014.