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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
02/08/2023 |
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Autoria: |
FISER, P. S.; AINSWORTH, L.; LANGFORD, G. A. |
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Título: |
Effect of osmolality of skimmMilk diluents and thawing rate on cryosurvival of ram spermatozoa. |
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Ano de publicação: |
1981 |
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Fonte/Imprenta: |
Cryobiology, v. 18, n. 4, p. 399-403, Aug. 1981. |
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DOI: |
10.1016/0011-2240(81)90113-9. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The effect of osmolality of skim-milk diluents (200, 320, 450, 600, and 750 mOsm/kg water) on the survival of ram spermatozoa frozen in straws were investigated after thawing in 39 °C water or in 20 °C air. Spermatozoa motility improved with increasing osmolality of the freezing diluent, irrespective of thawing rate. Diluents of 600 and 750 mOsm resulted in highest motility immediately after thawing and after 60 min incubation at 39 °C. A significant decrease in spermatozoa motility was observed when straws were thawed at 20 °C air with the magnitude of decrease inversely related to osmolality of the freezing diluent. Fertility of progestagen synchronized ewes inseminated with semen frozen in the 600 mOsm hypertonic skim-milk diluent was comparable to that obtained with fresh semen. |
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Palavras-Chave: |
Cell Survival; Osmolar Concentration; Semen preservation. |
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Thesagro: |
Ovino; Reprodução; Sêmen; Tecnologia. |
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Thesaurus Nal: |
Artificial insemination; Freezing; Reproduction; Sheep; Sperm motility; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01688naa a2200313 a 4500
001 1520029
005 2023-08-02
008 1981 bl uuuu u00u1 u #d
024 7 $a10.1016/0011-2240(81)90113-9.$2DOI
100 1 $aFISER, P. S.
245 $aEffect of osmolality of skimmMilk diluents and thawing rate on cryosurvival of ram spermatozoa.$h[electronic resource]
260 $c1981
520 $aAbstract: The effect of osmolality of skim-milk diluents (200, 320, 450, 600, and 750 mOsm/kg water) on the survival of ram spermatozoa frozen in straws were investigated after thawing in 39 °C water or in 20 °C air. Spermatozoa motility improved with increasing osmolality of the freezing diluent, irrespective of thawing rate. Diluents of 600 and 750 mOsm resulted in highest motility immediately after thawing and after 60 min incubation at 39 °C. A significant decrease in spermatozoa motility was observed when straws were thawed at 20 °C air with the magnitude of decrease inversely related to osmolality of the freezing diluent. Fertility of progestagen synchronized ewes inseminated with semen frozen in the 600 mOsm hypertonic skim-milk diluent was comparable to that obtained with fresh semen.
650 $aArtificial insemination
650 $aFreezing
650 $aReproduction
650 $aSheep
650 $aSperm motility
650 $aSpermatozoa
650 $aOvino
650 $aReprodução
650 $aSêmen
650 $aTecnologia
653 $aCell Survival
653 $aOsmolar Concentration
653 $aSemen preservation
700 1 $aAINSWORTH, L.
700 1 $aLANGFORD, G. A.
773 $tCryobiology$gv. 18, n. 4, p. 399-403, Aug. 1981.
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