01688naa a2200313 a 450000100080000000500110000800800410001902400390006010000170009924501230011626000090023952008120024865000280106065000130108865000170110165000100111865000190112865000160114765000100116365000170117365000110119065000150120165300180121665300260123465300230126070000180128370000200130177300530132115200292023-08-02       1981    bl uuuu     u00u1 u    #d7 a10.1016/0011-2240(81)90113-9.2DOI1 aFISER, P. S.  aEffect of osmolality of skimmMilk diluents and thawing rate on cryosurvival of ram spermatozoa.h[electronic resource]  c1981  aAbstract: The effect of osmolality of skim-milk diluents (200, 320, 450, 600, and 750 mOsm/kg water) on the survival of ram spermatozoa frozen in straws were investigated after thawing in 39 °C water or in 20 °C air. Spermatozoa motility improved with increasing osmolality of the freezing diluent, irrespective of thawing rate. Diluents of 600 and 750 mOsm resulted in highest motility immediately after thawing and after 60 min incubation at 39 °C. A significant decrease in spermatozoa motility was observed when straws were thawed at 20 °C air with the magnitude of decrease inversely related to osmolality of the freezing diluent. Fertility of progestagen synchronized ewes inseminated with semen frozen in the 600 mOsm hypertonic skim-milk diluent was comparable to that obtained with fresh semen.  aArtificial insemination  aFreezing  aReproduction  aSheep  aSperm motility  aSpermatozoa  aOvino  aReprodução  aSêmen  aTecnologia  aCell Survival  aOsmolar Concentration  aSemen preservation1 aAINSWORTH, L.1 aLANGFORD, G. A.  tCryobiologygv. 18, n. 4, p. 399-403, Aug. 1981.