| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Uva e Vinho. |
|
Data corrente: |
11/06/2019 |
|
Data da última atualização: |
06/01/2020 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
FALAVIGNA, V. da S.; MALABARBA, J.; SILVEIRA, C. P.; BUFFON, V.; MARIATH, J. E. de A.; PASQUALI, G.; MARGIS-PINHEIRO, M.; REVERS, L. F. |
|
Afiliação: |
Vítor da Silveira Falavigna, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; Jaiana Malabarba, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, BraziL; Carolina Pereira Silveira, Embrapa Uva e Vinho, Rua Livramento, 515, P.O. Box 130, Bento Gonçalves, RS 95701-008, Brazil; VANESSA BUFFON, CNPUV; Jorge Ernesto de Araújo Mariath, Laboratory of Plant Anatomy, Botany Department, UFRGS, Porto Alegre 91501-970, Brazil; Giancarlo Pasquali, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; Márcia Margis?Pinheiro, Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil; LUIS FERNANDO REVERS, CNPUV. |
|
Título: |
Characterization of the nucellus-specific dehydrin MdoDHN11 demonstrates its involvement in the tolerance to water deficit. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Plant Cell Reports [online], 2019. |
|
DOI: |
10.1007/s00299-019-02428-8 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywords Apple · Dehydrin · Seed development · Transgenic expression · Water-deficit stress MenosAbstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywor... Mostrar Tudo |
|
Palavras-Chave: |
Transgenic expression; Water-deficit stress. |
|
Thesaurus Nal: |
Apples; Dehydrins; Seed development. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198444/1/Falavigna2019-Article-CharacterizationOfTheNucellus-.pdf
|
|
Marc: |
LEADER 02425naa a2200277 a 4500
001 2109788
005 2020-01-06
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1007/s00299-019-02428-8$2DOI
100 1 $aFALAVIGNA, V. da S.
245 $aCharacterization of the nucellus-specific dehydrin MdoDHN11 demonstrates its involvement in the tolerance to water deficit.$h[electronic resource]
260 $c2019
520 $aAbstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywords Apple · Dehydrin · Seed development · Transgenic expression · Water-deficit stress
650 $aApples
650 $aDehydrins
650 $aSeed development
653 $aTransgenic expression
653 $aWater-deficit stress
700 1 $aMALABARBA, J.
700 1 $aSILVEIRA, C. P.
700 1 $aBUFFON, V.
700 1 $aMARIATH, J. E. de A.
700 1 $aPASQUALI, G.
700 1 $aMARGIS-PINHEIRO, M.
700 1 $aREVERS, L. F.
773 $tPlant Cell Reports [online], 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Uva e Vinho (CNPUV) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
09/11/2011 |
|
Data da última atualização: |
09/11/2011 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
SANTOS, F. H. DA R.; SILVEIRA, T. C. DA S.; SOARES, L. H. de B. |
|
Afiliação: |
FLÁVIA HERMELINA DA R. SANTOS, UFRRJ; TIANA CEZARINA DA S. SILVEIRA, UFRRJ; LUIS HENRIQUE DE BARROS SOARES, CNPAB. |
|
Título: |
Caracterização molecular de isolados bacterianos com habilidade celulolítica e avaliação do pré-tratamento de capim elefante por extrusão. |
|
Ano de publicação: |
2011 |
|
Fonte/Imprenta: |
SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 11., 2011, Seropédica. Mudanças climáticas, desastres naturais e prevenção de riscos: resumos... Seropédica: Embrapa Agrobiologia, 2011. |
|
Páginas: |
42 p. |
|
Idioma: |
Português |
|
Palavras-Chave: |
Acinetob acter; Biodecomposição; Extrução. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/45912/1/CARACTERIZACAO-MOLECULAR.pdf
|
|
Marc: |
LEADER 00729nam a2200169 a 4500
001 1905436
005 2011-11-09
008 2011 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. H. DA R.
245 $aCaracterização molecular de isolados bacterianos com habilidade celulolítica e avaliação do pré-tratamento de capim elefante por extrusão.$h[electronic resource]
260 $aSEMANA CIENTÍFICA JOHANNA DÖBEREINER, 11., 2011, Seropédica. Mudanças climáticas, desastres naturais e prevenção de riscos: resumos... Seropédica: Embrapa Agrobiologia$c2011
300 $a42 p.
653 $aAcinetob acter
653 $aBiodecomposição
653 $aExtrução
700 1 $aSILVEIRA, T. C. DA S.
700 1 $aSOARES, L. H. de B.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
