02425naa a2200277 a 450000100080000000500110000800800410001902400360006010000240009624501510012026000090027152015940028065000110187465000140188565000210189965300260192065300250194670000180197170000200198970000150200970000250202470000170204970000240206670000180209077300390210821097882020-01-06       2019    bl uuuu     u00u1 u    #d7 a10.1007/s00299-019-02428-82DOI1 aFALAVIGNA, V. da S.  aCharacterization of the nucellus-specific dehydrin MdoDHN11 demonstrates its involvement in the tolerance to water deficit.h[electronic resource]  c2019  aAbstract Key message MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development.AbstractDehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehy-dration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was local-ized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell.Keywords Apple · Dehydrin · Seed development · Transgenic expression · Water-deficit stress  aApples  aDehydrins  aSeed development  aTransgenic expression  aWater-deficit stress1 aMALABARBA, J.1 aSILVEIRA, C. P.1 aBUFFON, V.1 aMARIATH, J. E. de A.1 aPASQUALI, G.1 aMARGIS-PINHEIRO, M.1 aREVERS, L. F.  tPlant Cell Reports [online], 2019.